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J Liq Chromatogr Relat Technol. 2016 May 8;39(8):422-427. Epub 2016 Apr 22.

Simultaneous quantitative analysis of main components in linderae reflexae radix with one single marker.

Author information

1
School of Pharmacy, Henan University of Traditional Chinese Medicine, Jinshui District, ZhengZhou, China; Collaborative Innovation Center for Respiratory Disease Diagnosis and Treatment & Chinese Medicine Development of Henan Province, Jinshui District, ZhengZhou, China.
2
Henan Province Hospital of Traditional Chinese Medicine , Jinshui District , ZhengZhou , China.
3
School of Pharmacy, Henan University of Traditional Chinese Medicine , Jinshui District , ZhengZhou , China.

Abstract

Establish a quantitative analysis of multi-components by the single marker (QAMS) method for quality evaluation and validate its feasibilities by the simultaneous quantitative assay of four main components in Linderae Reflexae Radix. Four main components of pinostrobin, pinosylvin, pinocembrin, and 3,5-dihydroxy-2-(1-p-mentheneyl)-trans-stilbene were selected as analytes to evaluate the quality by RP-HPLC coupled with a UV-detector. The method was evaluated by a comparison of the quantitative results between the external standard method and QAMS with a different HPLC system. The results showed that no significant differences were found in the quantitative results of the four contents of Linderae Reflexae Radix determined by the external standard method and QAMS (RSD <3%). The contents of four analytes (pinosylvin, pinocembrin, pinostrobin, and Reflexanbene I) in Linderae Reflexae Radix were determined by the single marker of pinosylvin. This fingerprint was the spectra determined by Shimadzu LC-20AT and Waters e2695 HPLC that were equipped with three different columns.

KEYWORDS:

Linderae Reflexae Radix; Quantitative analysis multi-components by single marker; pinostrobin

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