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Essays Biochem. 2016 Jun 30;60(1):9-18. doi: 10.1042/EBC20150002.

Antibodies and antibody-derived analytical biosensors.

Author information

1
Biomedical Diagnostics Institute, Dublin City University, Dublin 9, Ireland.
2
School of Biotechnology, Dublin City University, Dublin 9, Ireland.
3
Biomedical Diagnostics Institute, Dublin City University, Dublin 9, Ireland School of Biotechnology, Dublin City University, Dublin 9, Ireland Richard.OKennedy@dcu.ie.

Abstract

The rapid diagnosis of many diseases and timely initiation of appropriate treatment are critical determinants that promote optimal clinical outcomes and general public health. Biosensors are now being applied for rapid diagnostics due to their capacity for point-of-care use with minimum need for operator input. Antibody-based biosensors or immunosensors have revolutionized diagnostics for the detection of a plethora of analytes such as disease markers, food and environmental contaminants, biological warfare agents and illicit drugs. Antibodies are ideal biorecognition elements that provide sensors with high specificity and sensitivity. This review describes monoclonal and recombinant antibodies and different immobilization approaches crucial for antibody utilization in biosensors. Examples of applications of a variety of antibody-based sensor formats are also described.

KEYWORDS:

antibodies; biosensor; monoclonal antibodies; recombinant antibodies

PMID:
27365031
PMCID:
PMC4986469
DOI:
10.1042/EBC20150002
[Indexed for MEDLINE]
Free PMC Article

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