Heat-treated Escherichia coli as a high-capacity biosorbent for tungsten anions

Bioresour Technol. 2016 Oct:218:140-5. doi: 10.1016/j.biortech.2016.06.076. Epub 2016 Jun 21.

Abstract

Adsorption performance in the biosorption of tungsten using Escherichia coli cells can be significantly improved by using cell suspensions that have been heat-treated at ⩽100°C. In the case of E. coli cells suspension heated at 100°C, the aqueous tungsten ions concentration rapidly decreased from 0.8mmol/L to practically zero within 1h. This biosorption time is much shorter than that of non-heat treated E. coli cells (7h). Furthermore, the adsorption saturation amount for cells heat-treated at 100°C was significantly increased up to 1.62mmol-W/g-E. coli compared to the unheated E. coli cells case (0.62mmol-W/g-E. coli). Determination of the surface potential and surface structure along with quantitative analyses of free amino acids of heat-treated E. coli cells were also carried out and revealed that heated cells have a high zeta potential and express a higher concentration of amino acids on the cell surface.

Keywords: Amino acid analysis; Biosorption; Recycling engineering; Tungsten; Zeta potential.

MeSH terms

  • Adsorption
  • Escherichia coli / chemistry*
  • Heating
  • Microscopy, Electron, Scanning
  • Spectroscopy, Fourier Transform Infrared
  • Surface Properties
  • Tungsten / chemistry*
  • Water / chemistry

Substances

  • Water
  • Tungsten