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Sci Rep. 2016 Jun 30;6:29058. doi: 10.1038/srep29058.

Increased circulating concentrations of mesencephalic astrocyte-derived neurotrophic factor in children with type 1 diabetes.

Author information

1
Institute of Biotechnology, University of Helsinki, Finland.
2
Division of Pharmaceutical Biosciences, Centre for Drug Research, University of Helsinki, Finland.
3
Children's Hospital, University of Helsinki and Helsinki University Central Hospital, Finland.
4
Research Programs Unit, Diabetes and Obesity, University of Helsinki, Finland.
5
Icosagen Ltd., Tartu, Estonia.
6
Folkhälsan Research Center, Helsinki, Finland.

Abstract

Mesencephalic astrocyte-derived neurotrophic factor (MANF) was recently shown to be essential for the survival and proliferation of pancreatic β-cells in mice, where deletion of MANF resulted in diabetes. The current study aimed at determining whether the concentration of circulating MANF is associated with the clinical manifestation of human type 1 diabetes (T1D). MANF expression in T1D or MANF levels in serum have not been previously studied. We developed an enzyme-linked immunosorbent assay (ELISA) for MANF and measured serum MANF concentrations from 186 newly diagnosed children and adolescents and 20 adults with longer-term T1D alongside with age-matched controls. In healthy controls the mean serum MANF concentration was 7.0 ng/ml. High MANF concentrations were found in children 1-9 years of age close to the diagnosis of T1D. The increased MANF concentrations were not associated with diabetes-predictive autoantibodies and autoantibodies against MANF were extremely rare. Patients with conspicuously high MANF serum concentrations had lower C-peptide levels compared to patients with moderate MANF concentrations. Our data indicate that increased MANF concentrations in serum are associated with the clinical manifestation of T1D in children, but the exact mechanism behind the increase remains elusive.

PMID:
27356471
PMCID:
PMC4928177
DOI:
10.1038/srep29058
[Indexed for MEDLINE]
Free PMC Article

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