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Rheumatology (Oxford). 2016 Oct;55(10):1889-900. doi: 10.1093/rheumatology/kew255. Epub 2016 Jun 28.

Resveratrol lowers synovial hyperplasia, inflammatory markers and oxidative damage in an acute antigen-induced arthritis model.

Author information

1
Laboratorio de Envejecimiento e Inflamación, Instituto de Investigación Biomédica de A Coruña, Sergas, A Coruña, España.
2
Laboratorio de Envejecimiento e Inflamación, Instituto de Investigación Biomédica de A Coruña, Sergas, A Coruña, España Laboratorio de Patología Osteoarticular, Centro de Investigaciones y de Estudios Avanzados del Instituto Politécnico Nacional Cinvestav-IPN, Distrito Federal, México.
3
Laboratorio de Envejecimiento e Inflamación, Instituto de Investigación Biomédica de A Coruña, Sergas, A Coruña, España Grupo de Terapia Celular e Ingeniería Tisular.
4
Unidad de Cirugía Experimental, Instituto de Investigación Biomédica de A Coruña.
5
Laboratorio de Investigación Osteoarticular y Envejecimiento, Complexo Hospitalario Universitario A Coruña-Sergas, A Coruña, España.
6
Laboratorio de Envejecimiento e Inflamación, Instituto de Investigación Biomédica de A Coruña, Sergas, A Coruña, España maria.jose.lopez.armada@sergas.es.

Abstract

OBJECTIVE:

The present study aimed to determine the protective effects of dietary supplementation with resveratrol (RSV) in an acute antigen-induced arthritis (AIA) model.

METHODS:

Rats were randomly divided into three groups: control, AIA and RSV-treated AIA group. RSV (12.5 mg/kg/day) was given orally for 8 weeks before induction of AIA and until the end of the experiment (48 h after intra-articular injection). The control and AIA animals were administered 100 μl of water. Results were evaluated by macroscopic observation, histopathology and immunohistochemistry for anti-PCNA, macrophages (CD68), T lymphocytes (CD3), monocyte chemoattractant protein-1 and 8-oxo-7,8-dihydro-2'-deoxyguanine (a marker of DNA damage). Cytokine-induced neutrophil chemoattractant-1 in serum and peroxidase activity in synovial tissue were measured using commercial kits.

RESULTS:

At the end of the study, RSV significantly reduced knee swelling. Likewise, the histological score of synovial tissue also reduced significantly. The arthritis-protective effects were associated with a significant decrease in PCNA, CD68, CD3 and monocyte chemoattractant protein-1 staining, as well as a reduction in serum concentrations of cytokine-induced neutrophil chemoattractant-1. RSV treatment also decreased the level of the marker of DNA damage, 8-oxo-7,8-dihydro-2'-deoxyguanine. Accordingly, peroxidase activity in the synovial tissue was up-regulated.

CONCLUSION:

Dietary supplementation with RSV lowers the main pathological hallmarks of RA disease in an acute model of AIA. RSV may represent a promising strategy in controlling the severity of RA.

KEYWORDS:

antioxidant; diet; inflammation; oxidative stress; resveratrol; rheumatoid arthritis; synovial tissue hyperplasia

PMID:
27354682
DOI:
10.1093/rheumatology/kew255
[Indexed for MEDLINE]

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