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Anticancer Res. 2016 Jul;36(7):3699-704.

Gene Expression in Lipopolysaccharide-treated Human Monocytes Following Interaction with Hepatic Cancer Cells.

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Department of Medical Technology, School of Life and Environmental Science, Azabu University, Sagamihara-shi, Kanagawa, Japan
Department of Integrated and Holistic Immunology, Faculty of Medicine, Kagawa University, Kita-gun, Kagawa, Japan Research Institute for Healthy Living, Niigata University of Pharmacy and Applied Life Sciences, Niitsu-shi, Niigata, Japan.



Monocytes migrate into the tissue where they differentiate into various types of macrophages with tissue-specific characteristics. When human monocytes are co-cultured with colon cancer cells they exhibit increased mRNA expression of angiogenesis- and signaling pathway-related genes; however, this increase is suppressed by pretreatment with low-dose lipopolysaccharide (LPS). Thus, LPS-treated human monocytes may be useful in suppressing tumor invasion and proliferation in colon cancer. However, it is suggested that the characteristics of tumor-associated macrophages may differ depending on the type of cancer. The function of human tumor-associated macrophages in hepatic cancer remains unclear. In this study, we investigated mRNA expression of various genes in LPS-treated human monocytes following interaction with hepatic cancer cells.


The human monocyte cell line THP-1 was treated with LPS and subsequently co-cultured with the human hepatic cancer cell line HepG2. mRNA expression of various factors were then analyzed using quantitative real-time polymerase chain reaction (PCR) and DNA microarray.


The mRNA expressions of monocyte chemotactic protein-1, vascular endothelial growth factor-A, tumor necrosis factor-α, interleukin (IL)-1β, IL-8, nuclear factor-κB, RelB, signal transducer and activator of transcription 3, IL-10 and transforming growth factor-β in THP-1 cells following interaction with HepG2 cells, were suppressed by pretreatment with LPS.


LPS-treated human monocytes may be useful in suppressing tumor invasion and proliferation of hepatic cancer, as well as colon cancer. The co-culture system of monocytes and cancer cells may be beneficial for evaluating antitumor effects in LPS-treated monocytes.


Monocyte; co-culture; hepatic cancer cell; lipopolysaccharide

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