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Pflugers Arch. 2016 Sep;468(9):1505-16. doi: 10.1007/s00424-016-1832-7. Epub 2016 Jun 28.

Deep insights: intravital imaging with two-photon microscopy.

Author information

1
Institute of Physiology, University of Regensburg, Universitätsstr. 31, 93040, Regensburg, Germany. Ina.schiessl@vkl.uni-regensburg.de.
2
Institute of Physiology, University of Regensburg, Universitätsstr. 31, 93040, Regensburg, Germany.

Abstract

Intravital multiphoton microscopy is widely used to assess the structure and function of organs in live animals. Although different tissues vary in their accessibility for intravital multiphoton imaging, considerable progress has been made in the imaging quality of all tissues due to substantial technical improvements in the relevant imaging components, such as optics, excitation laser, detectors, and signal analysis software. In this review, we provide an overview of the technical background of intravital multiphoton microscopy. Then, we note a few seminal findings that were made through the use of multiphoton microscopy. Finally, we address the technical limitations of the method and provide an outlook for how these limitations may be overcome through future technical developments.

KEYWORDS:

Intravital imaging; Labeling strategies; Multiphoton microscopy

PMID:
27352273
DOI:
10.1007/s00424-016-1832-7
[Indexed for MEDLINE]

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