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J Ethnopharmacol. 2016 Aug 22;190:362-71. doi: 10.1016/j.jep.2016.06.056. Epub 2016 Jun 25.

Chelidonium majus crude extract inhibits migration and induces cell cycle arrest and apoptosis in tumor cell lines.

Author information

1
College for Preschool Teachers, Cirila i Metodija 22, 37000 Krusevac, Serbia. Electronic address: milena.nikolic.1987@gmail.com.
2
College of Applied Technical Studies and Technology, Kosanciceva 36, 37000 Krusevac, Serbia. Electronic address: mladennikolic2603@yahoo.com.
3
Department of Immunology and Microbiology, Faculty of Medical Sciences, University of Kragujevac, Svetozara Markovica 69, 34000 Kragujevac, Serbia. Electronic address: dejan.baskic@gmail.com.
4
Department of Genetics, Faculty of Medical Sciences, University of Kragujevac, Svetozara Markovica 69, 34000 Kragujevac, Serbia. Electronic address: dtodorovic@medf.kg.ac.rs.
5
Department of Pathophysiology, University of Kragujevac, Svetozara Markovica 69, 34000 Kragujevac, Serbia. Electronic address: pdjurdjevic@sbb.rs.
6
Department of Biochemistry, Faculty of Medical Sciences, University of Kragujevac, Svetozara Markovica 69, 34000 Kragujevac, Serbia. Electronic address: zaricmilan@gmail.com.
7
Department of Biology and Ecology, Faculty of Science, University of Kragujevac, Radoja Domanovica 12, 34000 Kragujevac, Serbia. Electronic address: mstankovic@kg.ac.rs.
8
Department of Forensic Medicine, Faculty of Medical Sciences, University of Kragujevac, Svetozara Markovica 69, 34000 Kragujevac, Serbia. Electronic address: mtodorovickg@gmail.com.
9
Special Hospital for Internal Desease, Vojvode Misica 2, 11400 Mladenovac, Serbia. Electronic address: dr.damv@gmail.com.
10
Department of Immunology and Microbiology, Faculty of Medical Sciences, University of Kragujevac, Svetozara Markovica 69, 34000 Kragujevac, Serbia. Electronic address: suzana.popovic@medf.kg.ac.rs.

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE:

Chelidonium majus L (Papaveraceae) is widely used in alternative medicine for treatment of various disorders. Antitumor activities of alkaloids isolated from this plant have been reviewed, while there are only a few studies that examine properties of the whole extract.

AIM OF THE STUDY:

The aim of the present study was to investigate direct cytotoxic effects, as well as indirect antitumor effects of Chelidonium majus ethanolic extract against different tumor cell lines,.

MATERIALS AND METHODS:

MTT and SRB assays were performed to estimate cytotoxic effects of Chelidonium majus extract against human tumor cell lines A549, H460, HCT 116, SW480, MDA-MB 231 and MCF-7 and peripheral blood mononuclear cells from healthy individuals. Cell cycle analysis was performed by flow cytometry. Type of cell death induced by extract was determined by flow cytometry and cell morphology assessment. Inhibitory effect on migration of cancer cells was assessed by wound healing assay.

RESULTS:

Chelidonium majus extract showed selective time- and dose-dependent increase of cytotoxicity in all six cell lines, with individual cell line sensitivities. Extract promoted cell cycle arrest and induced apoptosis. Cotreatment with doxorubicin enhanced cytotoxicity of the drug. Also, inhibitory effect on migration was shown with non-toxic extract concentration.

CONCLUSIONS:

These results indicate possible usefulness of Chelidonium majus crude extract in antitumor therapy, whether through its direct cytotoxic effect, by prevention of metastasis, or as adjuvant therapy.

KEYWORDS:

Apoptosis; Cancer; Chelidonium majus L; Crude extract; Cytotoxicity

PMID:
27350008
DOI:
10.1016/j.jep.2016.06.056
[Indexed for MEDLINE]

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