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PLoS One. 2016 Jun 27;11(6):e0158174. doi: 10.1371/journal.pone.0158174. eCollection 2016.

Plasmodium falciparum Rab1A Localizes to Rhoptries in Schizonts.

Author information

1
School of BioSciences, University of Melbourne, Melbourne, VIC, 3010, Australia.
2
Laboratoire de Biologie Cellulaire Comparative des Apicomplexes, Institut Cochin, INSERM U1016, CNRS UMR 8104, Université Paris Descartes, 75014, Paris, France.

Abstract

Over-expression of a GFP-PfRab1A fusion protein in Plasmodium falciparum schizonts produces a punctate pattern of fluorescence typical of rhoptries, secretory organelles involved in host cell invasion. The GFP-positive bodies were purified by a combination of differential and density gradient centrifugation and their protein content determined by MS/MS sequencing. Consistent with the GFP rhoptry-like pattern of transgenic parasites, four of the 19 proteins identified have been previously described to be rhoptry-associated and another four are ER or ER-associated proteins. Confirmation that GFP-PfRab1A decorates rhoptries was obtained by its co-localization with Rap1 and Ron4 in late phase schizonts. We conclude that PfRab1A potentially regulates vesicular traffic from the endoplasmic reticulum to the rhoptries in Apicomplexa parasites.

PMID:
27348424
PMCID:
PMC4922565
DOI:
10.1371/journal.pone.0158174
[Indexed for MEDLINE]
Free PMC Article

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