Send to

Choose Destination
Toxicology. 1989 Jun 16;56(3):289-99.

Differential cytotoxicity of sodium arsenite in human fibroblasts and Chinese hamster ovary cells.

Author information

Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan, R.O.C.


Human skin fibroblast (HF) cells were approximately 10-fold more sensitive to sodium arsenite toxicity than Chinese hamster ovary (CHO) cells using the clonogenic assay. G1-phase CHO cells showed a 2-fold increase in the susceptibility to the toxic effects of sodium arsenite as compared to asynchronous CHO cells. The concentrations of sodium arsenite required to kill 50% of the cell population were correlated with the intracellular glutathione levels in asynchronous, G1-phase CHO, and asynchronous HF cells. Moreover, verapamil potentiated the cytotoxicity of sodium arsenite in CHO cells but not in HF cells. These results indicated that a verapamil-sensitive outward channel may be involved in detoxification of arsenic in CHO cells. Treatment with sodium arsenite resulted in a marked cell-cycle disturbance in CHO, but not in HF cells. Thus, CHO cells may take time to recover from sodium arsenite insult before progressing through the cell cycle. A different response of sodium arsenite in heat-shock protein synthesis in these 2 cell types was also revealed.

[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center