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Int J Environ Res Public Health. 2016 Jun 23;13(7). pii: E629. doi: 10.3390/ijerph13070629.

Nickel-Refining Fumes Induced DNA Damage and Apoptosis of NIH/3T3 Cells via Oxidative Stress.

Author information

1
Department of Occupational Health, College of Public Health, Harbin Medical University, Harbin 150000, China. wangyue5056@163.com.
2
Department of Occupational Health, College of Public Health, Harbin Medical University, Harbin 150000, China. wangshengyuan@163.com.
3
Department of Environmental Health, College of Public Health, Harbin Medical University, Harbin 150000, China. jiali1013@163.com.
4
Department of Occupational Health, College of Public Health, Harbin Medical University, Harbin 150000, China. sleepapple@126.com.
5
Department of Occupational Health, College of Public Health, Harbin Medical University, Harbin 150000, China. b_sonia@163.com.
6
Department of Occupational Health, College of Public Health, Harbin Medical University, Harbin 150000, China. nice.hd@163.com.
7
Department of Occupational Health, College of Public Health, Harbin Medical University, Harbin 150000, China. cuicui790913@163.com.
8
Department of Occupational Health, College of Public Health, Harbin Medical University, Harbin 150000, China. wuyonghui777@163.com.

Abstract

Although there have been numerous studies examining the toxicity and carcinogenicity of nickel compounds in humans and animals, its molecular mechanisms of action are not fully elucidated. In our research, NIH/3T3 cells were exposed to nickel-refining fumes at the concentrations of 0, 6.25, 12.50, 25, 50 and 100 μg/mL for 24 h. Cell viability, cell apoptosis, reactive oxygen species (ROS) level, lactate dehydrogenase (LDH) assay, the level of glutathione (GSH), activities of superoxide dismutase (SOD), catalase (CAT), and malondialdehyde (MDA) level were detected. The exposure of NIH/3T3 cells to nickel-refining fumes significantly reduced cell viability and induced cell apoptotic death in a dose-dependent manner. Nickel-refining fumes significantly increased ROS levels and induced DNA damage. Nickel-refining fumes may induce the changes in the state of ROS, which may eventually initiate oxidative stress, DNA damage and apoptosis of NIH/3T3 cells.

KEYWORDS:

DNA damage; NIH/3T3cell; apoptosis; nickel-refining fumes; oxidative stress

PMID:
27347984
PMCID:
PMC4962170
DOI:
10.3390/ijerph13070629
[Indexed for MEDLINE]
Free PMC Article

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