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Bio Protoc. 2015 Oct 20;5(20). pii: e1623.

Isolation and Culture of Human Endometrial Epithelial Cells and Stromal Fibroblasts.

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Center for Reproductive Sciences, Department of Obstetrics, Gynecology and Reproductive Sciences, University of California, San Francisco, USA.
Department of Urology, University of California, San Francisco, USA, and Gladstone Institute of Virology and Immunology, San Francisco, USA.


Purification and culture of endometrial epithelial cells (eEC) and stromal fibroblasts (eSF) from endometrial biopsies allows for downstream cell-specific in vitro studies. The utility of this protocol is the ease with which cells are purified without contamination from unwanted cell types, and the ability to use patient-paired eEC and eSF in experiments. These methods have been previously published, but here the protocol has been updated for maximum efficiency.

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