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Fungal Genet Biol. 2016 Sep;94:11-4. doi: 10.1016/j.fgb.2016.06.004. Epub 2016 Jun 21.

Spiral-based microfluidic device for long-term time course imaging of Neurospora crassa with single nucleus resolution.

Author information

1
Computational and Molecular Biology Laboratory, Department of Molecular and Cellular Physiology, University of Cincinnati, Cincinnati, OH 45267, USA; Microsystems and BioMEMS Laboratory, Department of Electrical Engineering and Computing Systems, University of Cincinnati, Cincinnati, OH 45221, USA.
2
Computational and Molecular Biology Laboratory, Department of Molecular and Cellular Physiology, University of Cincinnati, Cincinnati, OH 45267, USA.
3
Microsystems and BioMEMS Laboratory, Department of Electrical Engineering and Computing Systems, University of Cincinnati, Cincinnati, OH 45221, USA.
4
Computational and Molecular Biology Laboratory, Department of Molecular and Cellular Physiology, University of Cincinnati, Cincinnati, OH 45267, USA. Electronic address: christian.hong@uc.edu.

Abstract

Real-time imaging of fluorescent reporters plays a critical role in elucidating fundamental molecular mechanisms including circadian rhythms in the model filamentous fungus, Neurospora crassa. However, monitoring N. crassa for an extended period of time with single nucleus resolution is a technically challenging task due to hyphal growth that rapidly moves beyond a region of interest during microscopy experiments. In this report, we have proposed a two-dimensional spiral-based microfluidic platform and applied for monitoring the single-nucleus dynamics in N. crassa for long-term time course experiments.

KEYWORDS:

Circadian rhythm; Microfluidic device; Neurospora crassa

PMID:
27345439
DOI:
10.1016/j.fgb.2016.06.004
[Indexed for MEDLINE]

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