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Br J Dermatol. 2017 Jan;176(1):176-183. doi: 10.1111/bjd.14825. Epub 2016 Nov 26.

Cross-reactivity between methylisothiazolinone, octylisothiazolinone and benzisothiazolinone using a modified local lymph node assay.

Author information

1
National Allergy Research Centre, Department of Dermato-Allergology, Copenhagen University Hospital Gentofte, Kildegårds Allé, 2900 Hellerup, Denmark.
2
Department of Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
3
Department of Dermato-Allergology, Copenhagen University Hospital Gentofte, Kildegårds Allé, 2900 Hellerup, Denmark.
4
Department of Environmental Science, Aarhus University, Frederiksborgvej 399, 4000 Roskilde, Denmark.

Abstract

BACKGROUND:

In the light of the exceptionally high rates of contact allergy to the preservative methylisothiazolinone (MI), information about cross-reactivity between MI, octylisothiazolinone (OIT) and benzisothiazolinone (BIT) is needed.

OBJECTIVES:

To study cross-reactivity between MI and OIT, and between MI and BIT.

METHODS:

Immune responses to MI, OIT and BIT were studied in vehicle and MI-sensitized female CBA mice by a modified local lymph node assay. The inflammatory response was measured by ear thickness, cell proliferation of CD4+ and CD8+ T cells, and CD19+ B cells in the auricular draining lymph nodes.

RESULTS:

MI induced significant, strong, concentration-dependent immune responses in the draining lymph nodes following a sensitization phase of three consecutive days. Groups of MI-sensitized mice were challenged on day 23 with 0·4% MI, 0·7% OIT and 1·9% BIT - concentrations corresponding to their individual EC3 values. No statistically significant difference in proliferation of CD4+ and CD8+ T cells was observed between mice challenged with MI compared with mice challenged with BIT and OIT.

CONCLUSIONS:

The data indicate cross-reactivity between MI, OIT and BIT, when the potency of the chemical was taken into account in choice of challenge concentration. This means that MI-sensitized individuals may react to OIT and BIT if exposed to sufficient concentrations.

PMID:
27343839
DOI:
10.1111/bjd.14825
[Indexed for MEDLINE]

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