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J Appl Physiol (1985). 2016 Aug 1;121(2):457-65. doi: 10.1152/japplphysiol.01042.2015. Epub 2016 Jun 23.

Antiapoptotic effect of exercise training on ovariectomized rat hearts.

Author information

1
Graduate Institute of Chinese Medicine Science, China Medical University, Taichung, Taiwan; Graduate Institute of Basic Medical Science, China Medical University, Taichung, Taiwan; Department of Health and Nutrition Biotechnology, Asia University, Taichung, Taiwan;
2
Graduate Institute of Clinical Medical Science, China Medical University, Taichung, Taiwan;
3
Department of Physical Therapy, Graduate Institute of Rehabilitation Science, China Medical University, Taichung, Taiwan;
4
Department of Psychology, Asia University, Taichung, Taiwan;
5
Translational Medicine Research Center, China Medical University Hospital, Taichung, Taiwan; Graduate Institute of Immunology, China Medical University, Taichung, Taiwan; Center for Neuropsychiatry, Department of Neurology, China Medical University Hospital, Taichung, Taiwan;
6
Center of Sleep Medicine, Department of Physical Medicine and Rehabilitation, Chung-Shan Medical University Hospital, Chung-Shan Medical University, Taichung, Taiwan;
7
Department of Sports Sciences, University of Taipei, Taipei, Taiwan;
8
Graduate Institute of Chinese Medicine Science, China Medical University, Taichung, Taiwan;
9
Department of Physical Therapy, Graduate Institute of Rehabilitation Science, China Medical University, Taichung, Taiwan; Department of Occupational Therapy, Asia University, Taichung, Taiwan; and School of Rehabilitation Science, Shanghai University of TCM, Shanghai, China shinda@mail.cmu.edu.tw.

Abstract

The purpose of this study was to evaluate the effects of exercise training on cardiac Fas receptor-dependent and mitochondria-dependent apoptotic pathways in ovariectomized rats. Histopathological analysis, TUNEL assay, and Western blotting were performed on the excised hearts from three groups of Sprague-Dawley rats, which were divided into a sham-operated group, a bilaterally ovariectomized group (OVX), and a bilaterally ovariectomized group that underwent treadmill running exercise for 60 min/day, 5 sessions/wk, for 10 wk (OVX-EX). The abnormal myocardial architecture, cardiac trichome-stained fibrosis and cardiac TUNEL-positive apoptotic cells in ovariectomized rats improved after exercise training. The protein levels of tumor necrosis factor-α, tumor necrosis factor receptor 1, Fas ligand, Fas receptors, Fas-associated death domain, activated caspase-8 and activated caspase-3 (Fas receptor-dependent apoptotic pathways), as well as t-Bid, Bad, Bak, Bax, cytosolic cytochrome c, activated caspase-9, and activated caspase-3 (mitochondria-dependent apoptotic pathways) were decreased in the OVX-EX group compared with the OVX group. Exercise training suppressed ovariectomy-induced cardiac Fas receptor-dependent and mitochondria-dependent apoptotic pathways in ovariectomized rat models. These findings might indicate a new therapeutic effect for exercise training to prevent cardiac apoptosis in menopausal or bilaterally oophorectomized women.

KEYWORDS:

cardiac; caspase; cell death; menopause; treadmill training

PMID:
27339185
DOI:
10.1152/japplphysiol.01042.2015
[Indexed for MEDLINE]
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