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Appl Microbiol Biotechnol. 2016 Oct;100(19):8583-91. doi: 10.1007/s00253-016-7614-5. Epub 2016 Jun 23.

Responses of bacterial community and functional marker genes of nitrogen cycling to biochar, compost and combined amendments in soil.

Wu H1,2,3, Zeng G4,5, Liang J6,7, Chen J3, Xu J3, Dai J3, Li X1,2, Chen M1,2, Xu P1,2, Zhou Y1,2, Li F1,2, Hu L1,2, Wan J1,2.

Author information

1
College of Environmental Science and Engineering, Hunan University, Changsha, 410082, People's Republic of China.
2
Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha, 410082, People's Republic of China.
3
Changjiang River Scientific Research Institute, Wuhan, 430072, People's Republic of China.
4
College of Environmental Science and Engineering, Hunan University, Changsha, 410082, People's Republic of China. zgming@hnu.edu.cn.
5
Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha, 410082, People's Republic of China. zgming@hnu.edu.cn.
6
College of Environmental Science and Engineering, Hunan University, Changsha, 410082, People's Republic of China. liangjie@hnu.edu.cn.
7
Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha, 410082, People's Republic of China. liangjie@hnu.edu.cn.

Abstract

Biochar and compost are seen as two attractive waste management options and are used for soil amendment and pollution remediation. The interaction between biochar and composting may improve the potential benefits of biochar and compost. We investigated soil physicochemical properties, bacterial community, bacterial 16S rRNA, and functional marker genes of nitrogen cycling of the soil remedied with nothing (S), compost (SC), biochar (SB), a mixture of compost and biochar (SBC), composted biochar (SBced), and a composted mixture of biochar and biomass (SBCing). The results were that all amendments (1) increased the bacterial community richness (except SB) and SBCing showed the greatest efficiency; (2) increased the bacterial community diversity (SBCing > SBC > SC > SBced > SB > S); and (3) changed the gene copy numbers of 16S rRNA, nirK, nirS, and nosZ genes of bacteria, ammonia-oxidizing archaea (AOA), and ammonia-oxidizing bacteria (AOB). All amendments (except SB) could increase the gene copy number of 16S rRNA, and SBCing had the greatest efficiency. The changes of soil bacterial community richness and diversity and the gene copy numbers of 16S rRNA, nirK, nirS, nosZ, AOA, and AOB would affect carbon and nitrogen cycling of the ecosystem and also implied that BCing had the greatest efficiency on soil amendment.

KEYWORDS:

16S rRNA; Bacterial community; Biochar; Compost; Functional marker genes; Soil amendment

PMID:
27338575
DOI:
10.1007/s00253-016-7614-5
[Indexed for MEDLINE]

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