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Viruses. 2016 Jun 22;8(6). pii: E178. doi: 10.3390/v8060178.

Marburg Virus Reverse Genetics Systems.

Author information

1
Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Novel and Emerging Infectious Diseases, Greifswald-Insel Riems 17493, Germany. kristina.schmidt@fli.bund.de.
2
Department of Microbiology, School of Medicine, Boston University, 620 Albany Street, Boston, MA 02118, USA. muehlber@bu.edu.
3
National Emerging Infectious Diseases Laboratories (NEIDL), Boston University, 620 Albany Street, Boston, MA 02118, USA. muehlber@bu.edu.

Abstract

The highly pathogenic Marburg virus (MARV) is a member of the Filoviridae family and belongs to the group of nonsegmented negative-strand RNA viruses. Reverse genetics systems established for MARV have been used to study various aspects of the viral replication cycle, analyze host responses, image viral infection, and screen for antivirals. This article provides an overview of the currently established MARV reverse genetic systems based on minigenomes, infectious virus-like particles and full-length clones, and the research that has been conducted using these systems.

KEYWORDS:

Ebola virus; Marburg virus; biosafety level 4; filoviruses; full-length clones; minigenome; nonsegmented negative-sense RNA viruses; reverse genetics system; virus rescue; virus-like particles

PMID:
27338448
PMCID:
PMC4926198
DOI:
10.3390/v8060178
[Indexed for MEDLINE]
Free PMC Article

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