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PLoS One. 2016 Jun 23;11(6):e0158012. doi: 10.1371/journal.pone.0158012. eCollection 2016.

Developmental Decrease of Neuronal Chloride Concentration Is Independent of Trauma in Thalamocortical Brain Slices.

Author information

1
Department of Neurology, Division of Child Neurology, Massachusetts General Hospital, Boston, Massachusetts, United States of America.
2
Harvard Medical School, Boston, Massachusetts, United States of America.

Abstract

The intraneuronal chloride concentration ([Cl-]i) is paramount for determining the polarity of signaling at GABAA synapses in the central nervous system. Sectioning hippocampal brain slices increases [Cl-]i in the superficial layers. It is not known whether cutting trauma also increases [Cl-]i in the neocortex and thalamus, and whether the effects of trauma change during development. We used Cl- imaging to study the [Cl-]i vs. the distance from the cut surface in acute thalamocortical slices from mice at developmental ages ranging from post-natal day 5 (P5) to P20. We demonstrate: 1) [Cl-]i is higher in the most superficial areas in both neocortical and thalamic brain slices at all ages tested and, 2) there is a developmental decrease in [Cl-]i that is independent of acute trauma caused by brain slicing. We conclude that [Cl-]i has a developmental progression during P5-20 in both the neocortex and thalamus. However, in both brain regions and during development the neurons closest to the slicing trauma have an elevated [Cl-]i.

PMID:
27337272
PMCID:
PMC4919081
DOI:
10.1371/journal.pone.0158012
[Indexed for MEDLINE]
Free PMC Article

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