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Open Microbiol J. 2016 Apr 30;10:90-6. doi: 10.2174/1874285801610010090. eCollection 2016.

Effects of Yeast and Bacterial Commensals and Pathogens of the Female Genital Tract on the Transepithelial Electrical Resistance of HeLa Cells.

Author information

1
Laboratory of Experimental Neurophysiology, Department of Neurology, Aeginition Hospital, Medical School, National and Kapodistrian University of Athens, Greece.
2
Mycology Research Laboratory, Microbiology Department, Medical School, National and Kapodistrian University of Athens, Greece.
3
Department of Nursing, Faculty of Human Movement and Quality of Life Sciences, University of Peloponnese, Sparta, Greece.
4
Department of Computer Science and Biomedical Informatics, University of Thessaly, Greece.
5
Department of Biopathology and Clinical Microbiology, Aeginition Hospital, Medical School, National and Kapodistrian University of Athens, Greece.

Abstract

Commensals of the human body can shift to a pathogenic phase when the host immune system is impaired. This study aims to investigate the effect of seven yeast and two bacterial commensals and opportunistic pathogens isolated from blood and the female genital tract on the transepithelial electrical resistance (TER) of human cervical epithelial cell cultures (HeLa). The pathogens Candida tropicalis, C. parapsilosis, C. glabrata, C. krusei, C. albicans and Saccharomyces cerevisiae, caused a significant decrease in TER as compared to the controls; Lactobacillus spp caused a significant increase in TER versus the controls and Escherichia coli had no effect on the TER of the cell monolayers. The above data show that Candida spp., S. cerevisiae and Lactobacillus spp. have a non-selective effect on the TER of HeLa cell monolayers. These results are consistent with the in vivo non-selective action of these microorganisms on the various human mucosal epithelia.

KEYWORDS:

Candida; E. coli; HeLa cells; Lactobacillus; Saccharomyces; Transepithelial electrical resistance

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