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FASEB J. 2016 Oct;30(10):3334-3351. Epub 2016 Jun 22.

Warburg effect linked to cognitive-executive deficits in FMR1 premutation.

Author information

1
Department of Molecular Biosciences, School of Veterinary Medicine, University of California, Davis, Davis, California, USA.
2
Medical Investigations of Neurodevelopmental Disorders (MIND) Institute, University of California, Davis, Davis, California, USA; Department of Pediatrics, University of California Davis Medical Center, Sacramento California, USA; and.
3
Medical Investigations of Neurodevelopmental Disorders (MIND) Institute, University of California, Davis, Davis, California, USA.
4
Medical Investigations of Neurodevelopmental Disorders (MIND) Institute, University of California, Davis, Davis, California, USA; Department of Biochemistry and Molecular Medicine, School of Medicine, University of California, Davis, Davis, California, USA.
5
Department of Molecular Biosciences, School of Veterinary Medicine, University of California, Davis, Davis, California, USA; Department of Pediatrics, University of California Davis Medical Center, Sacramento California, USA; and cgiulivi@ucdavis.edu.

Abstract

A 55-200 CGG repeat expansion in the 5'-UTR of the fragile X mental retardation 1 (FMR1) gene is known as a premutation. Some carriers are affected by the neurodegenerative disorder fragile X-associated tremor/ataxia syndrome (FXTAS), primary ovarian insufficiency, and neurobehavioral impairments. Based on the mitochondrial dysfunction observed in fibroblasts and brain samples from carriers, as well as in neurons and brains from a mouse model of the premutation, we evaluated the presence of the Warburg effect in peripheral blood mononuclear cells (PBMCs) from 30 premutation carriers with either a rebalance of the metabolism [increasing glycolysis while decreasing oxidative phosphorylation (oxphos)] or a metabolic amplification (increasing glycolysis while maintaining/increasing oxphos). Deficits in oxphos-more pronounced in FXTAS-affected subjects-were accompanied by a shift toward glycolysis, suggesting increased glycolysis despite aerobic conditions. Differential proteomics extended these findings, unveiling a decreased antioxidant response, translation, and disrupted extracellular matrix and cytoskeleton organization with activation of prosenescence pathways. Lower bioenergetics segregated with increased incidence of low executive function, tremors, below-average IQ, and FXTAS. The combination of functional and proteomic data unveiled new mechanisms related to energy production in the premutation, showing the potential of being applicable to other psychiatric disorders to identify endophenotype-specific responses relevant to neurobiology.-Napoli, E., Song, G., Schneider, A., Hagerman, R., Eldeeb, M. A. A. A., Azarang, A., Tassone, F., Giulivi, C. Warburg effect linked to cognitive-executive deficits in FMR1 premutation.

KEYWORDS:

fragile X; mitochondria; neurological disorder; proteomics; triplet nucleotide diseases

PMID:
27335370
PMCID:
PMC5024697
DOI:
10.1096/fj.201600315R
[Indexed for MEDLINE]
Free PMC Article

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