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Genetics. 2016 Aug;203(4):1789-806. doi: 10.1534/genetics.116.192559. Epub 2016 Jun 22.

Facilitation of Endosomal Recycling by an IRG Protein Homolog Maintains Apical Tubule Structure in Caenorhabditis elegans.

Author information

  • 1Department of Molecular Biosciences, University of Kansas, Lawrence, Kansas 66045 Department of Biological Sciences, Minnesota State University, Mankato, Minnesota 56001.
  • 2Department of Molecular Biosciences, University of Kansas, Lawrence, Kansas 66045.
  • 3Department of Biological Sciences, Minnesota State University, Mankato, Minnesota 56001.
  • 4Mucosal Immunology and Biology Research Center, Developmental Biology and Genetics Core, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts 02114.
  • 5Department of Neuroscience, Center for Caenorhabditis elegans Anatomy, Albert Einstein College of Medicine, Bronx, New York 10461.
  • 6Department of Molecular Biosciences, University of Kansas, Lawrence, Kansas 66045 buechner@ku.edu.

Abstract

Determination of luminal diameter is critical to the function of small single-celled tubes. A series of EXC proteins, including EXC-1, prevent swelling of the tubular excretory canals in Caenorhabditis elegans In this study, cloning of exc-1 reveals it to encode a homolog of mammalian IRG proteins, which play roles in immune response and autophagy and are associated with Crohn's disease. Mutants in exc-1 accumulate early endosomes, lack recycling endosomes, and exhibit abnormal apical cytoskeletal structure in regions of enlarged tubules. EXC-1 interacts genetically with two other EXC proteins that also affect endosomal trafficking. In yeast two-hybrid assays, wild-type and putative constitutively active EXC-1 binds to the LIM-domain protein EXC-9, whose homolog, cysteine-rich intestinal protein, is enriched in mammalian intestine. These results suggest a model for IRG function in forming and maintaining apical tubule structure via regulation of endosomal recycling.

KEYWORDS:

IRG; endosomes; immunity-related GTPase; trafficking; tubulogenesis

PMID:
27334269
PMCID:
PMC4981278
[Available on 2017-08-01]
DOI:
10.1534/genetics.116.192559
[PubMed - in process]
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