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Appl Microbiol Biotechnol. 2016 Aug;100(15):6619-6630. doi: 10.1007/s00253-016-7677-3. Epub 2016 Jun 23.

Recent advances in the biotechnological production of microbial poly(ɛ-L-lysine) and understanding of its biosynthetic mechanism.

Xu Z1,2, Xu Z1,2, Feng X1,2, Xu D1,2, Liang J3, Xu H4,5.

Author information

1
State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing, 211816, China.
2
College of Food Science and Light Industry, Nanjing Tech University, Nanjing, 211816, China.
3
Nanjing Shineking Biological Technology Company, Nanjing, 210000, China.
4
State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing, 211816, China. xuh@njtech.edu.cn.
5
College of Food Science and Light Industry, Nanjing Tech University, Nanjing, 211816, China. xuh@njtech.edu.cn.

Abstract

Poly(ɛ-L-lysine) (ɛ-PL) is an unusual biopolymer composed of L-lysine connected between α-carboxyl and ɛ-amino groups. It has been used as a preservative in food and cosmetics industries, drug carrier in medicines, and gene carrier in gene therapy. Modern biotechnology has significantly improved the synthetic efficiency of this novel homopoly(amino acid) on an industrial scale and has expanded its industrial applications. In the latest years, studies have focused on the biotechnological production and understanding the biosynthetic mechanism of microbial ɛ-PL. Herein, this review focuses on the current trends and future perspectives of microbial ɛ-PL. Information on the screening of ɛ-PL-producing strains, fermentative production of ɛ-PL, breeding of high-ɛ-PL-producing strains, genomic data of ɛ-PL-producing strains, biosynthetic mechanism of microbial ɛ-PL, and the control of molecular weight of microbial ɛ-PL is included. This review will contribute to the development of this novel homopoly(amino acid) and serve as a basis of studies on other biopolymers.

KEYWORDS:

Biopolymer; Fermentation; Non-ribosomal peptide synthetase; Poly(ɛ-L-lysine); Streptomyces

PMID:
27333910
DOI:
10.1007/s00253-016-7677-3
[Indexed for MEDLINE]

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