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Nano Lett. 2016 Jul 13;16(7):4483-9. doi: 10.1021/acs.nanolett.6b01661. Epub 2016 Jun 27.

Measurement of DNA Translocation Dynamics in a Solid-State Nanopore at 100 ns Temporal Resolution.

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Department of Electrical Engineering, Columbia University , New York, New York 10027, United States.
Department of Physics and Astronomy, University of Pennsylvania , Philadelphia, Pennsylvania 19104, United States.
Department of Applied Physics and Applied Mathematics, Columbia University , New York, New York 10027, United States.
BGI-Shenzhen, Shenzhen, China.
School of Engineering, Brown University , Providence, Rhode Island 02912, United States.


Despite the potential for nanopores to be a platform for high-bandwidth study of single-molecule systems, ionic current measurements through nanopores have been limited in their temporal resolution by noise arising from poorly optimized measurement electronics and large parasitic capacitances in the nanopore membranes. Here, we present a complementary metal-oxide-semiconductor (CMOS) nanopore (CNP) amplifier capable of low noise recordings at an unprecedented 10 MHz bandwidth. When integrated with state-of-the-art solid-state nanopores in silicon nitride membranes, we achieve an SNR of greater than 10 for ssDNA translocations at a measurement bandwidth of 5 MHz, which represents the fastest ion current recordings through nanopores reported to date. We observe transient features in ssDNA translocation events that are as short as 200 ns, which are hidden even at bandwidths as high as 1 MHz. These features offer further insights into the translocation kinetics of molecules entering and exiting the pore. This platform highlights the advantages of high-bandwidth translocation measurements made possible by integrating nanopores and custom-designed electronics.


CMOS; DNA; Nanopore; high bandwidth; low noise amplifier

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