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Data Brief. 2016 Jun 3;8:404-10. doi: 10.1016/j.dib.2016.05.069. eCollection 2016 Sep.

Data on the characterization of follicle-stimulating hormone monoclonal antibodies and localization in Japanese eel pituitary.

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Aquaculture Research Division, National Institute of Fisheries Science (NIFS), Busan 46083, Republic of Korea.
Animal Biotechnology, Graduate School of Future Convergence Technology, Institute of Genetic Engineering, Hankyong National University, Anseong 17579, Republic of Korea.
Dept. of Research and Development, Institute of Gyeongbuk Marine Bioindustry, Ulgin, 36315, Republic of Korea.
Department of Food Science & Nutrition, Hoseo University, Asan 31499, Republic of Korea.


Monoclonal antibodies were generated against recombinant follicle-stimulating hormone (rec-FSH) from Japanese eel Anguilla japonica; rec-FSH was produced in Escherichia coli and purified using Ni-NTA Sepharose column chromatography. In support of our recent publication, "Production and characterization of monoclonal antibodies against recombinant tethered follicle-stimulating hormone from Japanese eel Anguilla japonica" [1], it was important to characterize the specificity of eel follicle-stimulating hormone antibodies. Here, the production and ELISA system of these monoclonal antibodies are presented. The affinity-purified monoclonal antibodies specifically detected eel rec-FSH in ELISA and on western blots of rec-FSH produced from CHO cells. Immunohistochemical analysis revealed that FSH staining was specifically localized in the eel pituitary.


FSH; Japanese eel; Monoclonal Antibody

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