Format

Send to

Choose Destination
Eur Radiol. 2017 Mar;27(3):1105-1113. doi: 10.1007/s00330-016-4457-5. Epub 2016 Jun 21.

Fluorescence molecular tomography of DiR-labeled mesenchymal stem cell implants for osteochondral defect repair in rabbit knees.

Author information

1
Department of Orthopaedic Sports Medicine, Klinikum rechts der Isar, Technische Universität München, Munich, Germany. Markus.Berninger@bgu-murnau.de.
2
Department of Trauma and Orthopaedic Surgery, BG Unfallklinik Murnau, Prof.-Küntscher-Strasse 8, 82418, Murnau, Germany. Markus.Berninger@bgu-murnau.de.
3
Institute for Biological and Medical Imaging, Technische Universität München und Helmholtz Zentrum München, Neuherberg, Germany.
4
Department of Radiology, Klinikum rechts der Isar, Technische Universität München, Munich, Germany.
5
Department of Orthopaedic Sports Medicine, Klinikum rechts der Isar, Technische Universität München, Munich, Germany.
6
Department of Clinical Radiology, Universitätsklinikum Münster, Münster, Germany.
7
Institute for Medical Statistics and Epidemiology, Klinikum rechts der Isar, Technische Universität München, Munich, Germany.
8
Institute for Experimental Oncology and Therapy Research, Klinikum rechts der Isar, Technische Universität München, Munich, Germany.
9
Section of Neuroradiology, Uniklinik Köln, Cologne, Germany.
10
Section of Neuroradiology, Krankenhaus der Barmherzigen Brüder, Trier, Germany.
11
Department of Diagnostic and Interventional Radiology, Universitätsklinikum Ulm, Ulm, Germany.

Abstract

OBJECTIVES:

To assess labelling efficiency of rabbit mesenchymal stem cells (MSCs) using the near-infrared dye 1,1'-dioctadecyl-3,3,3',3'-tetramethylindotricarbocyanine iodide (DiR) and detection of labelled MSCs for osteochondral defect repair in a rabbit model using fluorescence molecular tomography-X-ray computed tomography (FMT-XCT).

METHODS:

MSCs were isolated from New Zealand White rabbits and labelled with DiR (1.25-20 μg/mL). Viability and induction of apoptosis were assessed by XTT- and Caspase-3/-7-testing. Chondrogenic potential was evaluated by measurement of glycosaminoglycans. Labelled cells and unlabeled controls (n = 3) underwent FMT-XCT imaging before and after chondrogenic differentiation. Osteochondral defects were created surgically in rabbit knees (n = 6). Unlabeled and labelled MSCs were implanted in fibrin-clots and imaged by FMT-XCT. Statistical analyses were performed using multiple regression models.

RESULTS:

DiR-labelling of MSCs resulted in a dose-dependent fluorescence signal on planar images in trans-illumination mode. No significant reduction in viability or induction of apoptosis was detected at concentrations below 10 μg DiR/mL (p > .05); the chondrogenic potential of MSCs was not affected (p > .05). FMT-XCT of labelled MSCs in osteochondral defects showed a significant signal of the transplant (p < .05) with additional high-resolution anatomical information about its osteochondral integration.

CONCLUSIONS:

FMT-XCT allows for detection of stem cell implantation within osteochondral regeneration processes.

KEY POINTS:

• DiR-labelling of MSCs shows no toxic side effects or impairment of chondrogenesis. • Fluorescence molecular tomography allows for detection of MSCs for osteochondral defect repair. • FMT-XCT helps to improve evaluation of cell implantation and osteochondral regeneration processes.

KEYWORDS:

Cell labelling; Fluorescence molecular imaging; Mesenchymal stem cells; Osteochondral; Rabbit

PMID:
27329519
DOI:
10.1007/s00330-016-4457-5
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Springer
Loading ...
Support Center