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Cancer Res. 2016 Aug 15;76(16):4720-4727. doi: 10.1158/0008-5472.CAN-15-3134. Epub 2016 Jun 20.

CSN1 Somatic Mutations in Penile Squamous Cell Carcinoma.

Author information

1
UCL Cancer Institute, University College London, London, United Kingdom.
2
Centre for Molecular Oncology, Barts Cancer Institute, Queen Mary University of London, London, United Kingdom.
3
Division of Surgery and Interventional Science, UCL Medical School, University College London, London, United Kingdom.
4
Department of Urology, University College Hospital, London, United Kingdom.
5
Department of Urology, The Royal Surrey County Hospital, Surrey, United Kingdom.
6
Department of Urology, The Royal Berkshire NHS Foundation Trust, Reading, United Kingdom.
7
Department of Histopathology, University College London Hospital, London, United Kingdom.
8
Experimental Cancer Medicine Centre, Barts Cancer Institute, Barts Health and the Royal Free NHS Trust, Queen Mary University of London, London, United Kingdom.
9
NIHR Biomedical Research Centre, University College London Hospitals, London, United Kingdom.
#
Contributed equally

Abstract

Other than an association with HPV infection, little is known about the genetic alterations determining the development of penile cancer. Although penile cancer is rare in the developed world, it presents a significant burden in developing countries. Here, we report the findings of whole-exome sequencing (WES) to determine the somatic mutational landscape of penile cancer. WES was performed on penile cancer and matched germline DNA from 27 patients undergoing surgical resection. Targeted resequencing of candidate genes was performed in an independent 70 patient cohort. Mutation data were also integrated with DNA methylation and copy-number information from the same patients. We identified an HPV-associated APOBEC mutation signature and an NpCpG signature in HPV-negative disease. We also identified recurrent mutations in the novel penile cancer tumor suppressor genes CSN1(GPS1) and FAT1 Expression of CSN1 mutants in cells resulted in colocalization with AGO2 in cytoplasmic P-bodies, ultimately leading to the loss of miRNA-mediated gene silencing, which may contribute to disease etiology. Our findings represent the first comprehensive analysis of somatic alterations in penile cancer, highlighting the complex landscape of alterations in this malignancy. Cancer Res; 76(16); 4720-7.

PMID:
27325650
PMCID:
PMC5302160
DOI:
10.1158/0008-5472.CAN-15-3134
[Indexed for MEDLINE]
Free PMC Article

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