Depletion of autophagy receptor p62/SQSTM1 enhances the efficiency of gene delivery in mammalian cells

FEBS Lett. 2016 Aug;590(16):2671-80. doi: 10.1002/1873-3468.12262. Epub 2016 Jul 4.

Abstract

Novel methods that increase the efficiency of gene delivery to cells will have many useful applications. Here, we report a simple approach involving depletion of p62/SQSTM1 to enhance the efficiency of gene delivery. The efficiency of reporter gene delivery was remarkably higher in p62-knockout murine embryonic fibroblast (MEF) cells compared with normal MEF cells. This higher efficiency was partially attenuated by ectopic expression of p62. Furthermore, siRNA-mediated knockdown of p62 clearly increased the efficiency of transfection of murine embryonic stem (mES) cells and human HeLa cells. These data indicate that p62 acts as a key regulator of gene delivery.

Keywords: DNA beads; LC3; autophagy.

Publication types

  • Letter
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Fibroblasts / metabolism
  • Gene Knockout Techniques
  • Gene Transfer Techniques*
  • Genetic Therapy*
  • HeLa Cells
  • Humans
  • Mice
  • Mouse Embryonic Stem Cells / metabolism
  • RNA, Small Interfering / genetics
  • Sequestosome-1 Protein / antagonists & inhibitors
  • Sequestosome-1 Protein / genetics*
  • Transfection / methods*

Substances

  • RNA, Small Interfering
  • SQSTM1 protein, human
  • Sequestosome-1 Protein