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Development. 2016 Aug 1;143(15):2791-802. doi: 10.1242/dev.133686. Epub 2016 Jun 17.

Drosophila Condensin II subunit Chromosome-associated protein D3 regulates cell fate determination through non-cell-autonomous signaling.

Author information

1
Department of Cellular and Molecular Medicine, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, OH 44195, USA.
2
Bioinfo, Plantagenet, ON K0B 1L0, Canada.
3
Department of Cellular and Molecular Medicine, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, OH 44195, USA Department of Molecular Biosciences, Northwestern University, Evanston, IL 60201, USA.
4
Disease Mechanism Research Core, RIKEN Brain Science Institute, Wako, Saitama 351-0198, Japan.
5
Division of Biology, Kansas State University, Manhattan, KS 66506, USA.
6
Department of Cellular and Molecular Medicine, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, OH 44195, USA longwom@ccf.org.

Abstract

The pattern of the Drosophila melanogaster adult wing is heavily influenced by the expression of proteins that dictate cell fate decisions between intervein and vein during development. dSRF (Blistered) expression in specific regions of the larval wing disc promotes intervein cell fate, whereas EGFR activity promotes vein cell fate. Here, we report that the chromatin-organizing protein CAP-D3 acts to dampen dSRF levels at the anterior/posterior boundary in the larval wing disc, promoting differentiation of cells into the anterior crossvein. CAP-D3 represses KNOT expression in cells immediately adjacent to the anterior/posterior boundary, thus blocking KNOT-mediated repression of EGFR activity and preventing cell death. Maintenance of EGFR activity in these cells depresses dSRF levels in the neighboring anterior crossvein progenitor cells, allowing them to differentiate into vein cells. These findings uncover a novel transcriptional regulatory network influencing Drosophila wing vein development, and are the first to identify a Condensin II subunit as an important regulator of EGFR activity and cell fate determination in vivo.

KEYWORDS:

EGFR; KNOT; Serum response factor; Vein development; dCAP-D3; dSRF

PMID:
27317808
PMCID:
PMC5004906
DOI:
10.1242/dev.133686
[Indexed for MEDLINE]
Free PMC Article

Conflict of interest statement

The authors declare no competing or financial interests.

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