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Med Mycol. 2016 Nov 1;54(8):885-9. doi: 10.1093/mmy/myw044. Epub 2016 Jun 17.

Rapid identification of moulds and arthroconidial yeasts from positive blood cultures by MALDI-TOF mass spectrometry.

Author information

1
Central Laboratory Division-LIM03, Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, Brazil Laboratory of Medical Mycology - LIM-53, Hospital das Clínicas da FMUSP and Instituto de Medicina Tropical, Universidade de São Paulo, Brazil jnaj99@gmail.com.
2
Children's Institute, Hospital das Clínicas Faculdade de Medicina da Universidade de São Paulo, Brazil.
3
Central Laboratory Division-LIM03, Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, Brazil.
4
Laboratory of Bacteriology-LIM54, Instituto de Medicina Tropical, Universidade de São Paulo, Brazil.
5
Laboratory of Medical Mycology - LIM-53, Hospital das Clínicas da FMUSP and Instituto de Medicina Tropical, Universidade de São Paulo, Brazil.

Abstract

Moulds and arthroconidial yeasts are potential life-threatening agents of fungemia in immunocompromised patients. Fast and accurate identification (ID) of these pathogens hastens initiation of targeted antifungal therapy, thereby improving the patients' prognosis. We describe a new strategy that enabled the identification of moulds and arthroconidial yeasts directly from positive blood cultures by MALDI-TOF mass spectrometry (MS). Positive blood cultures (BCs) with Gram staining showing hyphae and/or arthroconidia were prospectively selected and submitted to an in-house protein extraction protocol. Mass spectra were obtained by Vitek MS™ system, and identifications were carried out with in the research use only (RUO) mode with an extended database (SARAMIS™ [v.4.12] plus in-house database). Fusarium solani, Fusarium verticillioides, Exophiala dermatitidis, Saprochaete clavata, and Trichosporon asahii had correct species ID by MALDI-TOF MS analysis of positive BCs. All cases were related to critically ill patients with high mortality fungemia and direct ID from positive BCs was helpful for rapid administration of targeted antifungal therapy.

KEYWORDS:

MALDI-TOF mass spectrometry; Mould; athroconidial yeasts; blood culture; fungemia

PMID:
27317582
DOI:
10.1093/mmy/myw044
[Indexed for MEDLINE]

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