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Methods Mol Biol. 2016;1439:1-32. doi: 10.1007/978-1-4939-3673-1_1.

Design and Implementation of High-Throughput Screening Assays.

Author information

1
Alternative Drug Discovery, GSK Pharmaceuticals, Gunnels Wood Road, Stevenage, Herts, SG1 2NY, UK. David.J.Powell@gsk.com.
2
GSK Pharmaceuticals R&D, Philadelphia, PA, USA.
3
Alternative Drug Discovery, GSK Pharmaceuticals, 1250 South Collegeville Road, Upper Providence, PA, 19426, USA.

Abstract

HTS remains at the core of the drug discovery process, and so it is critical to design and implement HTS assays in a comprehensive fashion involving scientists from the disciplines of biology, chemistry, engineering, and informatics. This requires careful consideration of many options and variables, starting with the choice of screening strategy and ending with the discovery of lead compounds. At every step in this process, there are decisions to be made that can greatly impact the outcome of the HTS effort, to the point of making it a success or a failure. Although specific guidelines should be established to ensure that the screening assay reaches an acceptable level of quality, many choices require pragmatism and the ability to compromise opposing forces.

KEYWORDS:

Bioassay; Drug screening; Human; Methodology; Phenotypic

PMID:
27316985
DOI:
10.1007/978-1-4939-3673-1_1
[Indexed for MEDLINE]

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