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Chem Commun (Camb). 2016 Jun 30;52(55):8545-8. doi: 10.1039/c6cc03098j.

A rapidly photo-activatable light-up fluorescent nucleoside and its application in DNA base variation sensing.

Author information

1
College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Wuhan, Hubei 430072, P. R. China. xzhou@whu.edu.cn dyh@whu.edu.cn.

Abstract

A new DNA building block (d(Tet)U) bearing a tetrazole and allyloxy group at N-phenyl ring linked through an aminopropynyl linker to the 5-position of 2'-deoxyuridine was synthesized. The modified DNA can be lit up via a photoinduced intramolecular tetrazole-alkene cycloaddition reaction, but quenched when the fully-matched double strand is formed. This conspicuous difference in fluorescence could open a door for DNA single nucleotide polymorphism (SNP) typing.

PMID:
27315545
DOI:
10.1039/c6cc03098j
[Indexed for MEDLINE]

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