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RNA Biol. 2017 May 4;14(5):488-499. doi: 10.1080/15476286.2016.1196320. Epub 2016 Jun 17.

Identification of miRNomes associated with adult neurogenesis after stroke using Argonaute 2-based RNA sequencing.

Author information

1
a Department of Neurology , Henry Ford Health System , Detroit , MI , USA.
2
b Sichuan Key Laboratory of Medical Imaging and Department of Immunology , North Sichuan Medical University , Nanchong , Sichuan , China.
3
c Department of Public Health Sciences , Henry Ford Health System , Detroit , MI , USA.
4
d Center for Bioinformatics , Henry Ford Health System , Detroit , MI , USA.
5
e Department of Radiology , Wayne State University , Detroit , MI , USA.
6
f Sichuan Key Laboratory of Medical Imaging and Department of Radiology , Affiliated Hospital of North Sichuan Medical University , Nanchong , Sichuan , China.
7
g Department of Physics , Oakland University , Rochester , MI , USA.

Abstract

Neurogenesis is associated with functional recovery after stroke. However, the underlying molecular mechanisms have not been fully investigated. Using an Ago2-based RNA immunoprecipitation to immunoprecipated Ago2-RNA complexes followed by RNA sequencing (Ago2 RIP-seq) approach, we profiled the miRNomes in neural progenitor cells (NPCs) harvested from the subventricular zone (SVZ) of the lateral ventricles of young adult rats. We identified more than 7 and 15 million reads in normal and ischemic NPC libraries, respectively. We found that stroke substantially changed Ago2-associated miRNA profiles in NPCs compared to those in non-ischemic NPCs. We also discovered a new complex repertoire of isomiRs and multiple miRNA-miRNA* pairs and numerous novel miRNAs in the non-ischemic and ischemic NPCs. Among them, pc-3p-17172 significantly regulated NPC proliferation and neuronal differentiation. Collectively, the present study reveals profiles of Ago2-associated miRNomes in non-ischemic and ischemic NPCs, which provide a molecular basis to further investigate the role of miRNAs in mediating adult neurogenesis under physiological and ischemic conditions.

KEYWORDS:

Ago2; RNA sequencing; microRNA; neural progenitor cells; neurogenesis; stroke

PMID:
27315491
PMCID:
PMC5449080
DOI:
10.1080/15476286.2016.1196320
[Indexed for MEDLINE]
Free PMC Article

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