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RNA Biol. 2016 Sep;13(9):820-5. doi: 10.1080/15476286.2016.1197481. Epub 2016 Jun 16.

PacBio full-length transcriptome profiling of insect mitochondrial gene expression.

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a College of Life Sciences, Nankai University , Tianjin, Tianjin , P.R. China.
b School of Mathematical Sciences, Nankai University , Tianjin , P.R.China.
c Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine , Tianjin , P.R. China.

Erratum in


In this study, we sequenced the first full-length insect transcriptome using the Erthesina fullo Thunberg based on the PacBio platform. We constructed the first quantitative transcription map of animal mitochondrial genomes and built a straightforward and concise methodology to investigate mitochondrial gene transcription, RNA processing, mRNA maturation and several other related topics. Most of the results were consistent with the previous studies, while to the best of our knowledge some findings were reported for the first time in this study. The new findings included the high levels of mitochondrial gene expression, the 3' polyadenylation and possible 5' m(7)G caps of rRNAs, the isoform diversity of 12S rRNA, the polycistronic transcripts and natural antisense transcripts of mitochondrial genes et al. These findings could challenge and enrich fundamental concepts of mitochondrial gene transcription and RNA processing, particularly of the rRNA primary (sequence) structure. The methodology constructed in this study can also be used to study gene expression or RNA processing of nuclear genomes.


Full-length transcriptome; PacBio; RNA processing; gene transcription; mitochondrial genes

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