Biopolymers Regulate Silver Nanoparticle under Microwave Irradiation for Effective Antibacterial and Antibiofilm Activities

Palaniyandi Velusamy; Chia-Hung Su; Govindarajan Venkat Kumar; Shritama Adhikary; Kannaiyan Pandian; Subash C B Gopinath; Yeng Chen; Periasamy Anbu

doi:10.1371/journal.pone.0157612

Biopolymers Regulate Silver Nanoparticle under Microwave Irradiation for Effective Antibacterial and Antibiofilm Activities

PLoS One. 2016 Jun 15;11(6):e0157612. doi: 10.1371/journal.pone.0157612. eCollection 2016.

Authors

Palaniyandi Velusamy¹, Chia-Hung Su², Govindarajan Venkat Kumar¹, Shritama Adhikary¹, Kannaiyan Pandian³, Subash C B Gopinath^{4

5}, Yeng Chen⁶, Periasamy Anbu⁷

Affiliations

¹ Department of Biotechnology, School of Bioengineering, SRM University, Kancheepuram, Tamil Nadu, India.
² Department of Chemical Engineering, Ming Chi University of Technology, Taishan, Taipei, Taiwan.
³ Department of Inorganic Chemistry, University of Madras, Guindy Campus, Chennai, Tamil Nadu, India.
⁴ Institute of Nano Electronic Engineering, Universiti Malaysia Perlis, Kangar, Perlis, Malaysia.
⁵ School of Bioprocess Engineering, Universiti Malaysia Perlis, Arau, Perlis, Malaysia.
⁶ Department of Oral Biology and Biomedical Sciences, Faculty of Dentistry, University of Malaya, Kuala Lumpur, Malaysia.
⁷ Department of Biological Engineering, College of Engineering, Inha University, Incheon, Republic of Korea.

Abstract

In the current study, facile synthesis of carboxymethyl cellulose (CMC) and sodium alginate capped silver nanoparticles (AgNPs) was examined using microwave radiation and aniline as a reducing agent. The biopolymer matrix embedded nanoparticles were synthesized under various experimental conditions using different concentrations of biopolymer (0.5, 1, 1.5, 2%), volumes of reducing agent (50, 100, 150 μL), and duration of heat treatment (30 s to 240 s). The synthesized nanoparticles were analyzed by scanning electron microscopy, UV-Vis spectroscopy, X-ray diffraction, and Fourier transform infrared spectroscopy for identification of AgNPs synthesis, crystal nature, shape, size, and type of capping action. In addition, the significant antibacterial efficacy and antibiofilm activity of biopolymer capped AgNPs were demonstrated against different bacterial strains, Staphylococcus aureus MTCC 740 and Escherichia coli MTCC 9492. These results confirmed the potential for production of biopolymer capped AgNPs grown under microwave irradiation, which can be used for industrial and biomedical applications.

MeSH terms

Anti-Bacterial Agents / chemistry*
Anti-Bacterial Agents / toxicity
Biofilms / drug effects
Biofilms / growth & development*
Biopolymers / chemistry*
Escherichia coli / drug effects
Escherichia coli / physiology
Metal Nanoparticles / chemistry*
Metal Nanoparticles / radiation effects
Metal Nanoparticles / toxicity
Microbial Sensitivity Tests
Microscopy, Electron, Scanning
Microwaves*
Particle Size
Silver / chemistry*
Spectrophotometry
Spectroscopy, Fourier Transform Infrared
Staphylococcus aureus / drug effects
Staphylococcus aureus / physiology
X-Ray Diffraction

Substances

Anti-Bacterial Agents
Biopolymers
Silver

Grants and funding

YC thanks the support from High Impact Research grants—“MoE Grant UM.C/625/1/HIR/MOE/DENT/09” and "UM-MoHE HIR UM.C/625/1/HIR/MOHE/MED/16/5". PA thanks Inha University, Republic of Korea, for providing a research grant. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.