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Cell Metab. 2016 Jun 14;23(6):1207-1215. doi: 10.1016/j.cmet.2016.05.023.

Quantification of GDF11 and Myostatin in Human Aging and Cardiovascular Disease.

Author information

1
Robert and Arlene Kogod Center on Aging, Mayo Clinic College of Medicine, Rochester, MN 55905, USA; Department of Physical Medicine and Rehabilitation, Mayo Clinic College of Medicine, Rochester, MN 55905, USA.
2
Division of Biomedical Statistics and Informatics, Department of Health Sciences Research, Mayo Clinic College of Medicine, Rochester, MN 55905, USA.
3
Medical Genome Facility-Proteomics Core, Mayo Clinic College of Medicine, Rochester, MN 55905, USA.
4
Center for Clinical and Translational Sciences, Mayo Clinic College of Medicine, Rochester, MN 55905, USA.
5
Robert and Arlene Kogod Center on Aging, Mayo Clinic College of Medicine, Rochester, MN 55905, USA.
6
Center for Innovation, Mayo Clinic College of Medicine, Rochester, MN 55905, USA.
7
Division of Cardiovascular Diseases, Department of Medicine, Mayo Clinic College of Medicine, Rochester, MN 55905, USA.
8
Division of Cardiovascular Surgery, Department of Medicine, Mayo Clinic College of Medicine, Rochester, MN 55905, USA.
9
Robert and Arlene Kogod Center on Aging, Mayo Clinic College of Medicine, Rochester, MN 55905, USA; Center for Clinical and Translational Sciences, Mayo Clinic College of Medicine, Rochester, MN 55905, USA; Division of Endocrinology, Department of Medicine, Mayo Clinic College of Medicine, Rochester, MN 55905, USA.
10
Robert and Arlene Kogod Center on Aging, Mayo Clinic College of Medicine, Rochester, MN 55905, USA; Division of Cardiovascular Surgery, Department of Medicine, Mayo Clinic College of Medicine, Rochester, MN 55905, USA.
11
Medical Genome Facility-Proteomics Core, Mayo Clinic College of Medicine, Rochester, MN 55905, USA; Department of Biochemistry and Molecular Biology, Mayo Clinic College of Medicine, Rochester, MN 55905, USA.
12
Robert and Arlene Kogod Center on Aging, Mayo Clinic College of Medicine, Rochester, MN 55905, USA; Department of Physical Medicine and Rehabilitation, Mayo Clinic College of Medicine, Rochester, MN 55905, USA. Electronic address: lebrasseur.nathan@mayo.edu.

Abstract

Growth and differentiation factor 11 (GDF11) is a transforming growth factor β superfamily member with a controversial role in aging processes. We have developed a highly specific LC-MS/MS assay to quantify GDF11, resolved from its homolog, myostatin (MSTN), based on unique amino acid sequence features. Here, we demonstrate that MSTN, but not GDF11, declines in healthy men throughout aging. Neither GDF11 nor MSTN levels differ as a function of age in healthy women. In an independent cohort of older adults with severe aortic stenosis, we show that individuals with higher GDF11 were more likely to be frail and have diabetes or prior cardiac conditions. Following valve replacement surgery, higher GDF11 at surgical baseline was associated with rehospitalization and multiple adverse events. Cumulatively, our results show that GDF11 levels do not decline throughout aging but are associated with comorbidity, frailty, and greater operative risk in older adults with cardiovascular disease.

PMID:
27304512
PMCID:
PMC4913514
DOI:
10.1016/j.cmet.2016.05.023
[Indexed for MEDLINE]
Free PMC Article

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