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Proc Natl Acad Sci U S A. 2016 Jun 28;113(26):7183-8. doi: 10.1073/pnas.1522381113. Epub 2016 Jun 14.

Maternally supplied S-acyl-transferase is required for crystalloid organelle formation and transmission of the malaria parasite.

Author information

1
Instituto de Medicina Molecular, Faculdade de Medicina da Universidade de Lisboa, 1649-028 Lisbon, Portugal;
2
Parasitology, Department of Infectious Diseases, University of Heidelberg Medical School, 69120 Heidelberg, Germany;
3
Leiden Malaria Research Group, Department of Parasitology, Leiden University Medical Center, 2333 ZA Leiden, The Netherlands;
4
Section on Electron Microscopy, Department of Molecular Cell Biology, Leiden University Medical Center, 2300 RC Leiden, The Netherlands;
5
Department of Pathogen Molecular Biology, Faculty of Infectious and Tropical Diseases, London School of Hygiene & Tropical Medicine, London WC1E 7HT, United Kingdom.
6
Instituto de Medicina Molecular, Faculdade de Medicina da Universidade de Lisboa, 1649-028 Lisbon, Portugal; Parasitology, Department of Infectious Diseases, University of Heidelberg Medical School, 69120 Heidelberg, Germany; gunnarmair@yahoo.com.

Abstract

Transmission of the malaria parasite from the mammalian host to the mosquito vector requires the formation of adequately adapted parasite forms and stage-specific organelles. Here we show that formation of the crystalloid-a unique and short-lived organelle of the Plasmodium ookinete and oocyst stage required for sporogony-is dependent on the precisely timed expression of the S-acyl-transferase DHHC10. DHHC10, translationally repressed in female Plasmodium berghei gametocytes, is activated translationally during ookinete formation, where the protein is essential for the formation of the crystalloid, the correct targeting of crystalloid-resident protein LAP2, and malaria parasite transmission.

KEYWORDS:

DOZI; crystalloid; malaria; ookinete; palmitoylation

PMID:
27303037
PMCID:
PMC4932936
DOI:
10.1073/pnas.1522381113
[Indexed for MEDLINE]
Free PMC Article

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