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Chem Commun (Camb). 2016 Jun 28;52(54):8405-8. doi: 10.1039/c6cc03119f.

Assessing the potential of photosensitizing flavoproteins as tags for correlative microscopy.

Author information

1
Madrid Institute for Advanced Studies in Nanoscience (IMDEA Nanoscience) and Nanobiotechnology Unit Associated to the National Center for Biotechnology (CSIC), 28049 Madrid, Spain. cristina.flors@imdea.org.
2
Madrid Institute for Advanced Studies in Nanoscience (IMDEA Nanoscience) and Nanobiotechnology Unit Associated to the National Center for Biotechnology (CSIC), 28049 Madrid, Spain. cristina.flors@imdea.org and CIC biomaGUNE, Paseo de Miramón 182, E-20009 Donostia-San Sebastian, Spain.
3
Institut Químic de Sarrià, Universitat Ramon Llull, 08017 Barcelona, Spain.

Abstract

Photosensitizing flavoproteins have great potential as tags for correlative light and electron microscopy (CLEM). We examine the photostability of miniSOG mutants and their ability to photo-oxidize diaminobenzidine, both key aspects for CLEM. Our experiments reveal a complex relation between these parameters and the production of different reactive oxygen species.

PMID:
27301706
DOI:
10.1039/c6cc03119f
[Indexed for MEDLINE]

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