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Plant Pathol J. 2016 Jun;32(3):273-80. doi: 10.5423/PPJ.NT.12.2015.0274. Epub 2016 Jun 1.

Biological Control Potential of Bacillus amyloliquefaciens KB3 Isolated from the Feces of Allomyrina dichotoma Larvae.

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Jeonnam Bioindustry Foundation, BioControl Research Center, Gokseong 57510, Korea.
Department of Biology, Utah State University, Logan, UT 843220-5305, USA.
Institute of Environmentally-Friendly Agriculture, Jeonnam National University, Gwangju 61186, Korea.


Most biocontrol agents for plant diseases have been isolated from sources such as soils and plants. As an alternative source, we examined the feces of tertiary larvae of the herbivorous rhino beetle, Allomyrina dichotoma for presence of biocontrol-active microbes. The initial screen was performed to detect antifungal activity against two common fungal plant pathogens. The strain with strongest antifungal activity was identified as Bacillus amyloliquefaciens KB3. The inhibitory activity of this strain correlated with lipopeptide productions, including iturin A and surfactin. Production of these surfactants in the KB3 isolate varied with the culture phase and growth medium used. In planta biocontrol activities of cell-free culture filtrates of KB3 were similar to those of the commercial biocontrol agent, B. subtilis QST-713. These results support the presence of microbes with the potential to inhibit fungal growth, such as plant pathogens, in diverse ecological niches.


Allomyrina dichotoma; biosurfactants; cyclic lipopeptides; growth medium; insect feces

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