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Virol J. 2016 Jun 14;13:101. doi: 10.1186/s12985-016-0545-z.

Classical swine fever virus NS5A protein changed inflammatory cytokine secretion in porcine alveolar macrophages by inhibiting the NF-κB signaling pathway.

Author information

1
College of Yingdong Agricultural Science and Engineering, Shaoguan University, Daxue Road, Zhenjiang District, Shaoguan, 512005, China.
2
North Guangdong Collaborative Innovation and Development Center for Swine Farming and Disease Control, Shaoguan, 512005, China.
3
College of Yingdong Agricultural Science and Engineering, Shaoguan University, Daxue Road, Zhenjiang District, Shaoguan, 512005, China. willertang@163.com.
4
North Guangdong Collaborative Innovation and Development Center for Swine Farming and Disease Control, Shaoguan, 512005, China. willertang@163.com.

Abstract

BACKGROUND:

Classical swine fever (CSF) caused by CSF virus (CSFV) is a highly contagious disease of the pigs. A number of studies have suggested that CSFV non-structural (NS) 5A protein is involved in CSFV-associated pathogenesis, but its mechanism is still uncertain. The aim of this study was to investigate the roles of NS5A protein in CSFV-associated pathogenesis in cultured porcine alveolar macrophages (PAMs).

METHODS:

After PAMs cultured in vitro were transfected with CSFV NS5A, the alterations in IL-1β, IL-6 and TNF-α expression were determined by ELISA, the RIG-I signaling activity related to inflammatory cytokine secretion was investigated by Western blot and Immunofluorescent staining.

RESULTS:

It was suggested that, the stable expressed CSFV NS5A solely had no influence on the expressions of inflammatory cytokines IL-1β, IL-6 and TNF-α in PAMs Moreover, NS5A protein could suppressed IL-1β, IL-6 and TNF-α expression induced by poly(I:C). It was also showed that NS5A protein did not impair the expressions of RIG-I, MDA5, IPS-1, NF-κB and IkBα in cells without poly(I:C) stimulation. Protein expressions of RIG-I, MDA5, IPS-1, NF-κB were not disrupted by NS5A protein in poly(I:C)-stimulated cells, while poly(I:C)-induced NF-κB nuclear translocation and activity was obviously suppressed by this protein. A suppression in poly(I:C)-induced IkBα degradation in NS5A-expressing cells was also observed.

CONCLUSION:

These data indicated that CSFV NS5A protein could inhibit the secretion of inflammatory cytokine induced by poly(I:C) through the suppression of the NF-κB signaling pathway, indicating the participation of CSFV NS5A protein in the pathogenesis of CSFV.

KEYWORDS:

CSFV; Cytokine; NF-κB; NS5A; RIG-I

PMID:
27296632
PMCID:
PMC4907015
DOI:
10.1186/s12985-016-0545-z
[Indexed for MEDLINE]
Free PMC Article

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