Format

Send to

Choose Destination
Int J Med Microbiol. 2016 Nov;306(7):580-585. doi: 10.1016/j.ijmm.2016.05.017. Epub 2016 May 28.

Substantial molecular evolution and mutation rates in prolonged latent Mycobacterium tuberculosis infection in humans.

Author information

1
International Reference Laboratory of Mycobacteriology, Statens Serum Institut, DK-2300 Copenhagen, Denmark. Electronic address: tll@ssi.dk.
2
Department of Systems Biology, Technical University of Denmark, DK-2800 Lyngby, Denmark.
3
International Reference Laboratory of Mycobacteriology, Statens Serum Institut, DK-2300 Copenhagen, Denmark.
4
Department of Infectious Diseases, Copenhagen University Hospital, Rigshospitalet, DK-2100 Copenhagen, Denmark.
5
Department of Systems Biology, Technical University of Denmark, DK-2800 Lyngby, Denmark. Electronic address: lj@bio.dtu.dk.

Abstract

The genome of Mycobacterium tuberculosis (Mtb) of latently infected individuals may hold the key to understanding the processes that lead to reactivation and progression to clinical disease. We report here analysis of pairs of Mtb isolates from putative prolonged latent TB cases. We identified two confirmed cases, and used whole genome sequencing to investigate the mutational processes that occur over decades in latent Mtb. We found an estimated mutation rate between 0.2 and 0.3 over 33 years, suggesting that latent Mtb accumulates mutations at rates similar to observations from cases of active disease.

KEYWORDS:

Genome sequencing; Latent infection; Mutation rate; Pathogen evolution; Within-host evolution

PMID:
27296510
DOI:
10.1016/j.ijmm.2016.05.017
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center