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Int J Biol Macromol. 2016 Oct;91:804-11. doi: 10.1016/j.ijbiomac.2016.05.111. Epub 2016 Jun 11.

Protective effects of polysaccharides from Psidium guajava leaves against oxidative stresses.

Author information

1
Department of Marine Life Science, Jeju National University, Jeju 690-756, Republic of Korea.
2
Jeju Aloe Agriculture Association Cooperation, Sehwa-ri, Pyosun-myeon, Seogwipo, Jeju-do 690-1, Republic of Korea.
3
Department of Polymer Science and Engineering, Sunchon National University, 255 Jungang-ro, Sunchon, Jeonnam 540-742, Republic of Korea.
4
Industry-Academic Cooperation Foundation, Jeju National University, Jeju 690-756, Republic of Korea.
5
Department of Food Science and Biotechnology, Kunsan National University, Kunsan 573-701, Republic of Korea.
6
Department of Polymer Science and Engineering, Sunchon National University, 255 Jungang-ro, Sunchon, Jeonnam 540-742, Republic of Korea. Electronic address: jwnah@sunchon.ac.kr.
7
Department of Marine Life Science, Jeju National University, Jeju 690-756, Republic of Korea. Electronic address: youjinj@jejunu.ac.kr.

Abstract

The aim of this study was to analyze antioxidant properties of a polysaccharide isolated from Psidium guajava leaves (PS-PGL) in vitro including its radical scavenging activities and protective effects against damage to cells as well as in vivo in zebrafish. The water extract of P. guajava leaves (WE-PGL) and PS-PGL showed strong radical scavenging effects in terms of 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl, and alkyl radical. Compared to WE-PGL, PS-PGL enhanced all scavenging activities and in particular strongly scavenged the hydroxyl radical (50% inhibitory concentration [IC50], 0.02mg/mL). In addition, PS-PGL exerted a protective effect against hydrogen peroxide-induced oxidative stress and against toxicity to Vero cells. Furthermore, in vivo experiments using zebrafish embryos indicated that treatment with hydrogen peroxide decreased the survival rate and heart-beating rate of zebrafish embryos, whereas these problems were reduced by PS-PGL treatment. Moreover, PS-PGL inhibited hydrogen peroxide-induced reactive oxygen species (ROS) production, lipid peroxidation, and cell death. Taken together, these results suggest that PS-PGL may be useful as a beneficial antioxidant material in the food and cosmetic industries.

KEYWORDS:

Antioxidant; Polysaccharides; Psidium guajava leaves

PMID:
27296444
DOI:
10.1016/j.ijbiomac.2016.05.111
[Indexed for MEDLINE]

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