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Int J Exerc Sci. 2016 Jan 15;9(1):34-46. eCollection 2016.

Leukocyte Subset Changes in Response to a 164-km Road Cycle Ride in a Hot Environment.

Author information

1
Department of Kinesiology, Health Promotion and Recreation, University of North Texas, Denton, TX, USA; Department of Biological Sciences, University of North Texas, Denton, TX, USA.
2
Department of Kinesiology, University of Connecticut, Storrs, CT, USA.
3
Department of Kinesiology, Health Promotion and Recreation, University of North Texas, Denton, TX, USA.

Abstract

The purpose of this observational study was to determine the circulating leukocyte subset response to completing the 2013 Hotter'N Hell Hundred recreational 164-km road cycle event in a hot and humid environmental condition. Twenty-eight men and four women were included in this study. Whole blood samples were obtained 1-2 hours before (PRE) and immediately after (POST) the event. Electronic sizing/sorting and cytometry were used to determine complete blood counts (CBC) including neutrophil, monocyte, and lymphocyte subsets. The concentration of circulating total leukocytes (103·μL-1) increased 134% from PRE to POST with the greatest increase in neutrophils (319%, p<0.0001). Circulating monocytes (including macrophages) increased 24% (p=0.004) and circulating lymphocytes including B and T cells increased 53% (p<0.0001). No association was observed between rolling time or relative intensity and leukocyte subset. Completing the Hotter n' Hell Hundred (HHH), a 100 mile recreational cycling race in extreme (hot and humid) environmental conditions, induces a substantial increase in total leukocytes in circulation. The contribution of increases in specific immune cell subsets is not equal, with neutrophils increasing to greater than 4-fold starting values from PRE to POST race. It is likely that exercise in stressful environmental conditions affects the complement of circulating immune cells, although activational state and characterization of specific leukocyte subsets remains unclear. The observed increase in circulating cell sub-populations suggests that the circulating immune surveillance system may be acutely affected by exercise in hot and humid conditions.

KEYWORDS:

White blood cells; exercise; heat; lymphocyte; monocyte; neutrophils; ultra-endurance

PMID:
27293505
PMCID:
PMC4882474

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