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Mol Cell. 2016 Jul 7;63(1):97-109. doi: 10.1016/j.molcel.2016.05.010. Epub 2016 Jun 9.

Cutoff Suppresses RNA Polymerase II Termination to Ensure Expression of piRNA Precursors.

Author information

1
California Institute of Technology, Division of Biology, 147-75, 1200 E. California Blvd., Pasadena, CA 91125, USA.
2
Biochemistry Center Regensburg, Laboratory for RNA Biology, University of Regensburg, 93053 Regensburg, Germany.
3
Memorial Sloan-Kettering Cancer Center, Structural Biology Program, 1275 York Avenue, New York, NY, 10021 USA.
4
Department of Molecular Genetics, University of Toronto, 1 King's College Circle, Toronto, Ontario, Canada M5S 1A8.
5
Department of Biochemistry, University of Toronto, 1 King's College Circle, Toronto, Ontario, Canada M5S 1A8.
#
Contributed equally

Abstract

Small non-coding RNAs called piRNAs serve as guides for an adaptable immune system that represses transposable elements in germ cells of Metazoa. In Drosophila the RDC complex, composed of Rhino, Deadlock and Cutoff (Cuff) bind chromatin of dual-strand piRNA clusters, special genomic regions, which encode piRNA precursors. The RDC complex is required for transcription of piRNA precursors, though the mechanism by which it licenses transcription remained unknown. Here, we show that Cuff prevents premature termination of RNA polymerase II. Cuff prevents cleavage of nascent RNA at poly(A) sites by interfering with recruitment of the cleavage and polyadenylation specificity factor (CPSF) complex. Cuff also protects processed transcripts from degradation by the exonuclease Rat1. Our work reveals a conceptually different mechanism of transcriptional enhancement. In contrast to other factors that regulate termination by binding to specific signals on nascent RNA, the RDC complex inhibits termination in a chromatin-dependent and sequence-independent manner.

PMID:
27292797
PMCID:
PMC4980073
DOI:
10.1016/j.molcel.2016.05.010
[Indexed for MEDLINE]
Free PMC Article

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