Format

Send to

Choose Destination
Appl Microbiol Biotechnol. 2016 Nov;100(21):9091-9102. Epub 2016 Jun 10.

Optimization of nonribosomal peptides production by a psychrotrophic fungus: Trichoderma velutinum ACR-P1.

Author information

1
Fermentation Technology Division, CSIR-Indian Institute of Integrative Medicine, Jammu, 180001, India.
2
Academy of Scientific and Innovative Research, New Delhi, 110001, India.
3
Medicinal Chemistry Division, CSIR-Indian Institute of Integrative Medicine, Jammu, 180001, India.
4
Faculty of Sciences, Department of Biotechnology, Shri Mata Vaishno Devi University, Katra, 182320, India.
5
Medicinal Chemistry Division, CSIR-Indian Institute of Integrative Medicine, Jammu, 180001, India. dsingh@iiim.ac.in.
6
Academy of Scientific and Innovative Research, New Delhi, 110001, India. dsingh@iiim.ac.in.
7
Fermentation Technology Division, CSIR-Indian Institute of Integrative Medicine, Jammu, 180001, India. achaubey@iiim.ac.in.
8
Academy of Scientific and Innovative Research, New Delhi, 110001, India. achaubey@iiim.ac.in.

Abstract

Trichoderma is an anamorphic filamentous fungal genus with immense potential for production of small valuable secondary metabolites with indispensable biological activities. Microbial dynamics of a psychrotrophic strain Trichoderma velutinum ACR-P1, isolated from unexplored niches of the Shiwalik region, bestowed with rich biodiversity of microflora, was investigated for production of nonribosomal peptides (NRPs) by metabolite profiling by intact-cell mass spectrometry (ICMS) employing matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometer. Being the first report on NRPs production by T. velutinum, studies on optimization of growth conditions by Response Surface Methodology (RSM) for production of NRPs by ACR-P1 was carried out strategically. Multifold enhancement in the yield of NRPs belonging to subfamily SF4 with medium chain of amino acid residues having m/z 1437.9, 1453.9, and 1452.0 at pH 5.9 at 20 °C and of subfamily SF1 with long-chain amino acid residues having m/z 1770.2, 1784.2, 1800.1, 1802.1, and 1815.1 was achieved at pH 7.0 at 25 °C. Complexities of natural mixtures were thus considerably reduced under respective optimized culture conditions accelerating the production of novel microbial natural products by saving time and resources.

KEYWORDS:

ICMS; MALDI-TOF MS; Nonribosomal peptides (NRPs); Optimization; RSM; Shiwalik region; Trichoderma velutinum

PMID:
27287849
DOI:
10.1007/s00253-016-7622-5
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Springer
Loading ...
Support Center