Format

Send to

Choose Destination
Nat Commun. 2016 Jun 10;7:11705. doi: 10.1038/ncomms11705.

In vivo imaging of clock gene expression in multiple tissues of freely moving mice.

Author information

1
Department of Applied Molecular-Imaging Physics, Hokkaido University Graduate School of Medicine, Sapporo 060-8638, Japan.
2
Department of Medical Physics and Engineering, Hokkaido University Graduate School of Medicine, Sapporo 060-8638, Japan.
3
Department of Biomedical Science and Engineering, Hokkaido University Graduate School of Health Science, Sapporo 062-0812, Japan.
4
Global Station for Quantum Medical Science and Engineering, Global Institution for Collaborative Research and Education (GI-CoRE), Hokkaido University, Sapporo 060-8638, Japan.
5
Department of Chronomedicine, Hokkaido University Graduate School of Medicine, Sapporo 060-8638, Japan.
6
Department of Radiation Medicine, Hokkaido University Graduate School of Medicine, Sapporo 060-8638, Japan.

Abstract

Clock genes are expressed throughout the body, although how they oscillate in unrestrained animals is not known. Here, we show an in vivo imaging technique that enables long-term simultaneous imaging of multiple tissues. We use dual-focal 3D tracking and signal-intensity calibration to follow gene expression in a target area. We measure circadian rhythms of clock genes in the olfactory bulb, right and left ears and cortices, and the skin. In addition, the kinetic relationship between gene expression and physiological responses to experimental cues is monitored. Under stable conditions gene expression is in phase in all tissues. In response to a long-duration light pulse, the olfactory bulb shifts faster than other tissues. In Cry1(-/-) Cry2(-/-) arrhythmic mice circadian oscillation is absent in all tissues. Thus, our system successfully tracks circadian rhythms in clock genes in multiple tissues in unrestrained mice.

PMID:
27285820
PMCID:
PMC5446038
DOI:
10.1038/ncomms11705
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Nature Publishing Group Icon for PubMed Central
Loading ...
Support Center