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Science. 2016 Jun 10;352(6291):1326-1329. doi: 10.1126/science.aaf6463.

Oligodendrocyte heterogeneity in the mouse juvenile and adult central nervous system.

Author information

1
Laboratory of Molecular Neurobiology, Department Medical Biochemistry and Biophysics, Karolinska Institutet, SE-17177 Stockholm, Sweden.
2
Department of Physiology and Pharmacology, Karolinska Institutet SE-17177 Stockholm, Sweden.
3
Wolfson Institute for Biomedical Research, University College London, Gower Street, London WC1E 6BT, United Kingdom.
4
Institute of Neuroscience, Second Military Medical University, 800 Xiangyin Road, Shanghai 200433, China.
5
Department of Molecular Neurosciences, Center for Brain Research, Medical University of Vienna, Austria.
#
Contributed equally

Abstract

Oligodendrocytes have been considered as a functionally homogeneous population in the central nervous system (CNS). We performed single-cell RNA sequencing on 5072 cells of the oligodendrocyte lineage from 10 regions of the mouse juvenile and adult CNS. Thirteen distinct populations were identified, 12 of which represent a continuum from Pdgfra(+) oligodendrocyte precursor cells (OPCs) to distinct mature oligodendrocytes. Initial stages of differentiation were similar across the juvenile CNS, whereas subsets of mature oligodendrocytes were enriched in specific regions in the adult brain. Newly formed oligodendrocytes were detected in the adult CNS and were responsive to complex motor learning. A second Pdgfra(+) population, distinct from OPCs, was found along vessels. Our study reveals the dynamics of oligodendrocyte differentiation and maturation, uncoupling them at a transcriptional level and highlighting oligodendrocyte heterogeneity in the CNS.

PMID:
27284195
PMCID:
PMC5221728
DOI:
10.1126/science.aaf6463
[Indexed for MEDLINE]
Free PMC Article

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