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Biochem Soc Trans. 2016 Jun 15;44(3):877-82. doi: 10.1042/BST20160054.

Artificial membranes for membrane protein purification, functionality and structure studies.

Author information

1
Astbury Centre for Structural Molecular Biology, School of Biomedical Sciences, University of Leeds, Leeds LS2 9JT, U.K. Biomedicine Research Group, Faculty of Health and Social Sciences, Leeds Beckett University, Leeds LS1 3HE, U.K.
2
Centre for Plant Sciences, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, U.K. Biomedicine Research Group, Faculty of Health and Social Sciences, Leeds Beckett University, Leeds LS1 3HE, U.K.
3
Astbury Centre for Structural Molecular Biology, School of Biomedical Sciences, University of Leeds, Leeds LS2 9JT, U.K.
4
Astbury Centre for Structural Molecular Biology, School of Biomedical Sciences, University of Leeds, Leeds LS2 9JT, U.K. Division of Biochemistry, Department of Biosciences, University of Helsinki, Helsinki FIN-00014, Finland.
5
Astbury Centre for Structural Molecular Biology, School of Biomedical Sciences, University of Leeds, Leeds LS2 9JT, U.K. Biomedicine Research Group, Faculty of Health and Social Sciences, Leeds Beckett University, Leeds LS1 3HE, U.K. v.l.postis@leedsbeckett.ac.uk.

Abstract

Membrane proteins represent one of the most important targets for pharmaceutical companies. Unfortunately, technical limitations have long been a major hindrance in our understanding of the function and structure of such proteins. Recent years have seen the refinement of classical approaches and the emergence of new technologies that have resulted in a significant step forward in the field of membrane protein research. This review summarizes some of the current techniques used for studying membrane proteins, with overall advantages and drawbacks for each method.

KEYWORDS:

bicelle; black lipid membrane (BLM); lipidic cubic phase (LCP); lipodisq; membrane proteins; nanodiscs; styrene maleic acid lipid particle system (SMALPs); supported lipid bilayer (SLB)

PMID:
27284055
DOI:
10.1042/BST20160054
[Indexed for MEDLINE]
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