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PLoS One. 2016 Jun 9;11(6):e0156498. doi: 10.1371/journal.pone.0156498. eCollection 2016.

Microarray Noninvasive Neuronal Seizure Recordings from Intact Larval Zebrafish.

Meyer M1,2,3,4, Dhamne SC1,2,4, LaCoursiere CM1,2,3, Tambunan D1,2,3, Poduri A1,2,3, Rotenberg A1,2,4.

Author information

1
Division of Epilepsy and Clinical Neurophysiology, Department of Neurology, Boston Children's Hospital, Harvard Medical School, Boston, Massachusetts, United States of America.
2
F.M. Kirby Neurobiology Center, Department of Neurology, Boston Children's Hospital, Harvard Medical School, Boston, Massachusetts, United States of America.
3
Epilepsy Genetics Program, Neurogenetics Program, Department of Neurology, Boston Children's Hospital, Harvard Medical School, Boston, Massachusetts, United States of America.
4
Neuromodulation Program, Department of Neurology, Boston Children's Hospital, Harvard Medical School, Boston, Massachusetts, United States of America.

Abstract

Zebrafish epilepsy models are emerging tools in experimental epilepsy. Zebrafish larvae, in particular, are advantageous because they can be easily genetically altered and used for developmental and drug studies since agents applied to the bath penetrate the organism easily. Methods for electrophysiological recordings in zebrafish are new and evolving. We present a novel multi-electrode array method to non-invasively record electrical activity from up to 61 locations of an intact larval zebrafish head. This method enables transcranial noninvasive recording of extracellular field potentials (which include multi-unit activity and EEG) to identify epileptic seizures. To record from the brains of zebrafish larvae, the dorsum of the head of an intact larva was secured onto a multi-electrode array. We recorded from individual electrodes for at least three hours and quantified neuronal firing frequency, spike patterns (continuous or bursting), and synchrony of neuronal firing. Following 15 mM potassium chloride- or pentylenetetrazole-infusion into the bath, spike and burst rate increased significantly. Additionally, synchrony of neuronal firing across channels, a hallmark of epileptic seizures, also increased. Notably, the fish survived the experiment. This non-invasive method complements present invasive zebrafish neurophysiological techniques: it affords the advantages of high spatial and temporal resolution, a capacity to measure multiregional activity and neuronal synchrony in seizures, and fish survival for future experiments, such as studies of epileptogenesis and development.

PMID:
27281339
PMCID:
PMC4900632
DOI:
10.1371/journal.pone.0156498
[Indexed for MEDLINE]
Free PMC Article

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