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Sci Rep. 2016 Jun 9;6:27623. doi: 10.1038/srep27623.

Multiplex giant magnetoresistive biosensor microarrays identify interferon-associated autoantibodies in systemic lupus erythematosus.

Author information

1
Department of Mechanical Engineering, Stanford University, Stanford, California, USA.
2
Department of Medicine, Division of Immunology and Rheumatology, Stanford University, Stanford, California, USA.
3
Department of Molecular and Cell Biology, Division of Immunology and Pathogenesis, University of California, Berkeley, California, USA.
4
Department of Electrical and Computer Engineering, University of California, San Diego, California, USA.
5
Division of Rheumatology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
6
Center for Immunology, University of Minnesota Medical School, Minneapolis, Minnesota, USA.
7
Department of Pediatrics, Division of Allergy, Immunology and Rheumatology, Stanford University, Stanford, California, USA.
8
Institute for Immunity, Transplantation and Infection, Stanford University School of Medicine, Stanford, California, USA.
9
Department of Materials Science and Engineering, Stanford University, Stanford, California, USA.
10
Department of Electrical Engineering, Stanford School of Engineering, Stanford, California, USA.

Abstract

High titer, class-switched autoantibodies are a hallmark of systemic lupus erythematosus (SLE). Dysregulation of the interferon (IFN) pathway is observed in individuals with active SLE, although the association of specific autoantibodies with chemokine score, a combined measurement of three IFN-regulated chemokines, is not known. To identify autoantibodies associated with chemokine score, we developed giant magnetoresistive (GMR) biosensor microarrays, which allow the parallel measurement of multiple serum antibodies to autoantigens and peptides. We used the microarrays to analyze serum samples from SLE patients and found individuals with high chemokine scores had significantly greater reactivity to 13 autoantigens than individuals with low chemokine scores. Our findings demonstrate that multiple autoantibodies, including antibodies to U1-70K and modified histone H2B tails, are associated with IFN dysregulation in SLE. Further, they show the microarrays are capable of identifying autoantibodies associated with relevant clinical manifestations of SLE, with potential for use as biomarkers in clinical practice.

PMID:
27279139
PMCID:
PMC4899742
DOI:
10.1038/srep27623
[Indexed for MEDLINE]
Free PMC Article

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