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BMC Infect Dis. 2016 Jun 9;16:260. doi: 10.1186/s12879-016-1615-y.

High-resolution melting analysis for molecular detection of multidrug resistance tuberculosis in Peruvian isolates.

Author information

1
Laboratorio de Biotecnología y Biología Molecular, Instituto Nacional de Salud, Lima, Peru.
2
Laboratorio de Referencia Regional de Tuberculosis y Microbiología, DIRESA Callao, Lima, Peru.
3
Laboratorio de Biotecnología y Biología Molecular, Instituto Nacional de Salud, Lima, Peru. hguio@ins.gob.pe.

Abstract

BACKGROUND:

The emergence of multidrug-resistant strains is a major health problem especially for countries with high TB incidence such as Peru. In this study, we evaluated High Resolution Melting (HRM) assay in Peruvian isolates for the detection of mutations within rpoB, katG genes and promoter region inhA to determine isoniazid and rifampicin resistance in Mycobacterium tuberculosis (Mtb).

METHODS:

DNA samples extracted from a total of 167 clinical isolates of Mtb, 89 drug-sensitive and 78 multidrug-resistant, were blindly analyzed by HRM analysis and verified by DNA sequencing.

RESULTS:

The HRM analysis generated patterns that were specific to distinguish between sensitive and resistance isolates. The sensitivity and specificity of the HRM assays in comparison with drug susceptibility testing (DST) for detection of rifampicin resistance were 98.7 % and 97.5 %, and for isoniazid resistance were 98.7 % and 100 %.

CONCLUSION:

This study suggests that HRM Analysis could help with rapid diagnosis of MDR-TB cases in Peru.

KEYWORDS:

High Resolution Melting Analysis; Multi-drug resistance tuberculosis; Tuberculosis

PMID:
27278526
PMCID:
PMC4899911
DOI:
10.1186/s12879-016-1615-y
[Indexed for MEDLINE]
Free PMC Article

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